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本文采用电泳技术,用血痕检测方法对云南省富源县水族人群的红细胞EsD和PGM1作了表型分布调查,计算出这两种同工酶的基因频率及个人识别能力。调查结果表明,红细胞EsD和PGM1在水族中均有较好的分布频率和较高的鉴别能力,并存在着不同程度的民族间差异。酯酶D(EsD)和磷酸葡萄糖变位酶—1(PGM1)均存在于人类红细胞上及某些组织中,它们的遗传标记按孟德尔分离律遗传。并且在人类的不同种族与群体中其基因频率分布不同,因此受到人类群体遗传学和法医学研究领域的关注,由于EsD及PGM1具有高度的多态性。作为重要的遗传标记,EsD和PGM1在个人识别,亲子鉴定等法医学领域里也已得到广泛的应用。国内对少数民族红细胞酶型作过一些调查,但云南省水族酶型频率分布调查尚未见报道。为了解EsD和PGM1在我国各民族人群中的分布状况,本文采用淀粉/琼脂糖混合凝胶电泳技术,用血痕检验方法,对云南省富源县水族人群进行了抽样调查,并就调查结果作了一些分析。
In this paper, using electrophoresis technology, using bloodstain detection method to investigate the phenotypic distribution of erythrocyte EsD and PGM1 in Fuyuan County of Yunnan Province, and to calculate the genetic frequency and personal identification ability of these two isozymes. The results show that the erythrocyte EsD and PGM1 in the aquarium have a better distribution frequency and higher discriminatory power, and there are varying degrees of inter-ethnic differences. Esterase D (EsD) and phosphoglucomutase-1 (PGM1) are present on human erythrocytes and in certain tissues, and their genetic markers are inherited by Mendel’s law of segregation. And its gene frequency distribution varies among different races and groups in humans and is therefore of interest in the field of genetics and forensic science in human populations due to the high degree of polymorphism of EsD and PGM1. As an important genetic marker, EsD and PGM1 have also been widely used in forensic sciences such as personal identification and paternity testing. Domestic ethnic erythrocyte enzyme made some investigations, but Yunnan Province, the investigation of the frequency of aquarium enzymes has not been reported. In order to understand the distribution of EsD and PGM1 in the ethnic groups in our country, this paper uses the starch / agarose gel electrophoresis technique to test the aquarium population in Fuyuan County of Yunnan Province by using the bloodstain test method. Some analysis.