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以复制缺陷型重组腺病毒载体(AdCMV-GFP)为对照,用复制缺陷型p53重组腺病毒载体 (AdCMV-p53)转染经0．5、1．0、2．0Gyγ射线照射的HT-29细胞,克隆形成法检测对细胞的抑制作用,流式细胞分析法检测细胞周期和细胞凋亡,探讨辐射诱导对AdCMV-p53转染p53突变型结直肠癌细胞(HT-29细胞系)细胞周期的影响。结果显示,0．5-1．0Gy辐射诱导明显增强40 MOI AdCMV-p53转染对HT-29细胞的抑制。与AdCMV-p53转染对照相比,1 d后,辐射诱导转染组G0／G1期细胞减少5%-15％,S期细胞增加 2%-19％,2．0Gy辐射诱导80 MOI AdCMV-p53转染组G2/M期细胞增加12％;3d后,0．5、1．0Gy辐射诱导40 MOI AdCMV-p53转染组G2／M期细胞分别增加10%-13％。辐射诱导AdCMV-p53转染组细胞凋亡与辐射诱导剂量和AdCMV-p53转染剂量相关。以上结果表明,辐射诱导加速AdCMV-p53转染细胞由G0／G1期到S期的进程,促进S期阻滞和G2/M期阻滞发生。
Using replication-deficient recombinant adenovirus vector (AdCMV-GFP) as a control, HT-29 cells transfected with 0.5, 1.0 and 2.0 Gy gamma rays were transfected with replication-defective p53 recombinant adenovirus vector (AdCMV-p53) The cell cycle and apoptosis were detected by flow cytometry (FCM). The effects of radiation on the cell cycle of transfected p53 mutant colorectal cancer cells (HT-29 cell line) by AdCMV-p53 Impact. The results showed that 0.5-1.0 Gy irradiation significantly enhanced the inhibition of HT-29 cells by 40 MOI AdCMV-p53 transfection. Compared with the AdCMV-p53 transfected control, the cells in the G0 / G1 phase decreased by 5% -15% and the S phase increased by 2% -19% after 1 d of radiation-induced transfection. The 2.0 Gy irradiation induced 80 MOI of AdCMV- The cells in G2 / M phase increased by 12% in p53 transfection group. After 3d, cells in G2 / M phase increased by 10% -13% in 40 MOI AdCMV-p53 transfected group with 0.5 and 1.0Gy radiation respectively. Radiation-induced apoptosis in AdCMV-p53 transfected cells was correlated with radiation-induced dose and AdCMV-p53 transfection dose. The above results showed that radiation induced accelerated progression of G0 / G1 phase to S phase in AdCMV-p53 transfected cells, which promoted S phase arrest and G2 / M arrest.