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目的 :观察由编码 HBs Ag与 HCV- CE2 抗原的两种重组真核细胞表达质粒制备的 DNA疫苗重复联合接种 BAL B/c小鼠后 ,其诱生的特异性免疫应答反应。方法 :应用上述两种 NDA疫苗重复联合免疫小鼠 ,动态观察血中特异性抗体水平、特异性细胞毒性 T淋巴细胞 (CTL )体外杀伤活性 ,并进行 CTL杀伤活性活体诱生实验。结果 :两种 DNA疫苗联合重复免疫小鼠 ,能够诱生机体特异性体液免疫及细胞免疫完全应答。其小鼠荷瘤表达目的抗原的靶细胞后 ,生存率明显高于未免疫鼠 (P <0 .0 5 )。结论 :乙型及丙型肝炎联合 DNA疫苗的重复接种 ,可有效地诱生小鼠机体特异性免疫应答反应。 DNA疫苗诱生的 CTL应答可与体液免疫应答分离存在 ,可能是 DNA疫苗免疫保护的更重要方面。
OBJECTIVE: To observe the specific immune response induced by repeatedly inoculating BALB / c mice with DNA vaccine prepared from two recombinant eukaryotic expression plasmids encoding HBs Ag and HCV-CE2 antigens. Methods: The combined immunized mice were repeatedly immunized with the above two kinds of NDA vaccine to observe the level of specific antibody in blood and cytotoxic T lymphocyte (CTL) in vitro. The CTL activity in vivo was also measured. Results: The two DNA vaccines combined with repeated immunization of mice induced body-specific humoral and cellular immune responses. The survival rate of mice bearing tumor cells expressing target antigen was significantly higher than that of non-immunized mice (P <0.05). Conclusion: Repeated inoculation of Hepatitis B and C combined with DNA vaccine can effectively induce the body-specific immune response in mice. CTL responses induced by DNA vaccines can be separated from the humoral immune response and may be a more important aspect of DNA vaccine immunoprotection.