自从Zenkteler等报道了六倍体小麦与玉米杂交形成圆球形幼胚的现象以来,D.A. Laurie发现,由于在最初的合子细胞分裂过程中,玉米染色体快速消失,结果形成了小麦单倍体幼胚,这样的幼胚活力很低,很快会退化消失。D. A.Laurie等用小穗培养法,从706个授粉后小麦小花中获得了47个单倍体幼胚,其中31个经胚抢救后成苗。由于产生的单倍体苗频率太低,难以用于小麦育种研究。为了提高单倍体苗获得率,扩大试验材料的基因型范围,我们探讨了不同方法 Since Zenkteler et al. Reported the phenomenon of hexaploid wheat crossing globular immature embryos with maize, DA Laurie found that due to the rapid disappearance of maize chromosomes during the initial zygote division, the haploid embryos of wheat were formed, This immature embryo is very low in energy and will soon degenerate and disappear. D. A. Laurie et al. Used spikelet culture to obtain 47 haploid embryos from 706 pollinated wheat florets, of which 31 were embryo-rescued. The frequency of haploid seedlings produced is too low to be used in wheat breeding research. In order to increase the haploid seedling yield and expand the genotype range of the test materials, we explored different methods