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1 材料与方法1.1 动物及分组 选用健康、雄性Wistar大鼠(由黑龙江中医药大学实验动物室提供)34只,体重为100g～200g,随机分成4组;空白组5只,模型组5只,治疗组12只,对照组12只。1.2 造模方法 模型组:将大鼠用乙醚麻醉后,固定在手术台上,用1‰新洁尔灭消毒下腹部皮毛,用镊子提起皮肤,用剪子剪开下腹部皮肤,逐层分离,找到前列腺。用注射器针头刺破大鼠前列腺,再用白金针蘸取大肠杆菌菌株(黑龙江中医药大学微生物教研室提供),从刺破处接种到大鼠前列腺中,接种后逐层缝合大鼠腹部。空白组:手术操作同模型组,但不再做细菌接种。
1 Materials and methods 1.1 Animals and subgroups Thirty-four healthy and male Wistar rats (provided by Laboratory Animal Room of Heilongjiang University of Traditional Chinese Medicine) weighing 100g to 200g were randomly divided into 4 groups; 5 blank groups and 5 model groups. Twelve treatment groups and 12 control groups. 1.2 Modeling method Model group: The rats were anesthetized with ether and fixed on the operating table. The lower abdomen fur was disinfected with 1 quinoline and the skin was lifted with tweezers. The lower abdomen skin was cut with scissors and separated layer by layer to find the prostate. The rat’s prostate was punctured with a syringe needle, and the E. coli strain was drawn with a white gold needle (provided by the Department of Microbiology, Heilongjiang University of Traditional Chinese Medicine). The rat prostate was inoculated from the puncture site and the rat’s abdomen was sutured layer by layer after inoculation. Blank group: The operation was the same as the model group, but no bacterial inoculation was performed.