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目的研究叶下珠Phyllanthus urinaria全草的化学成分。方法应用硅胶、Sephadex LH-20、RP_(18)、MCI-gel柱色谱和半制备HPLC等色谱方法对叶下珠的乙醇提取物进行分离纯化;根据理化性质和波谱学方法鉴定化合物的结构。结果从叶下珠95%乙醇提取物的石油醚和醋酸乙酯萃取部位中分离得到16个化合物,分别鉴定为没食子酸(1)、咖啡酸(2)、没食子酸乙酯(3)、没食子酸甲酯(4)、4-乙氧基苯甲酸(5)、邻苯二甲酸二异丁酯(6)、邻苯二甲酸二丁酯(7)、(4R,6R)-2,3-dihydromenisdaurilide(8)、(4R,6S)-2,3-dihydromenisdaurilide(9)、aquilegiolide(10)、menisdaurilide(11)、cassipourol(12)、(9Z,12Z)-nonadeca-9,12-dienoic acid(13)、亚油酸甲酯(14)、豆甾醇(15)、(8R,8’S,7S)-4’-(3"-methoxyrhamnopyranosyl)oxy-8’-hydroxy-3,3’,4-trimethoxy-8-hydroxymethyl-lign-7,9’-lactone(16)。结论化合物5~7、10、11、13、14为首次从该植物中分离得到,化合物8、9、12、16为首次从该属植物中分离得到。
Objective To study the chemical constituents of Phyllanthus urinaria. Methods The ethanol extracts of Phyllanthus urinaria were isolated and purified by chromatography on silica gel, Sephadex LH-20, RP_ (18), MCI-gel and semi-preparative HPLC. The structures of the compounds were identified by physicochemical and spectroscopic methods. Results Sixteen compounds were isolated from petroleum ether and ethyl acetate extracts of 95% ethanol extract of Phyllanthus urinaria and identified as gallic acid (1), caffeic acid (2), ethyl gallate (3) and gallic acid (4), 4-ethoxybenzoic acid (5), diisobutyl phthalate (6), dibutyl phthalate (7), (4R, 6R) -2,3 (4R, 6S) -2,3-dihydromenisalurilide (9), aquilegiolide (10), menisdaurilide (11), cassipourol (12), (9Z, 12Z) -nonadeca-9,12-dienoic acid (13), methyl linoleate (14), stigmasterol (15), (8R, 8’S, 7S) -4’- (3’-methoxyrhamnopyranosyl) oxy-8’-hydroxy- -trimethoxy-8-hydroxymethyl-lign-7,9’-lactone (16) .CONCLUSION Compounds 5-7, 10, 11, 13 and 14 were isolated from this plant for the first time and compounds 8, 9, Times from the genus isolated.