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探讨胆红素神经毒性脑组织中兴奋性氨基酸 (EAA)神经递质变化。方法 :制作胆红素脑病动物模型 ,在体脑内微透析提取神经突触间细胞外液 ,高效液相色谱 (HPLC)检测分析天冬氨酸 (Asp)谷氨酸 (Glu)甘氨酸 (Gly)。结果显示胆红素毒性脑组织细胞外 Gly含量为 2 1 .35±4.87μM,明显高于对照组 1 4 .0 2± 1 .56μM;胆红素毒性脑组织细胞外 Asp、Glu与对照组比较差异不显著。结论 :胆红素毒性脑组织细胞外甘氨酸 (Gly)含量增加 ,可提高 NMDA受体对 EAA神经递质 Asp、Glu敏感性 ,致 NMDA受体过度活化
To investigate the changes of neurotransmitter of excitatory amino acids (EAA) in bilirubin neurotoxicity brain tissue. Methods: Animal model of bilirubin encephalopathy was made. The extracellular synaptosomes were extracted by intracerebral microdialysis. The levels of Asp, Glu and Gly were determined by high performance liquid chromatography (HPLC) ). The results showed that the extracellular Gly content of bilirubin-toxic brain tissue was 21.35 ± 4.87μM, which was significantly higher than that of the control group (14.02 ± 1.56μM) The difference is not significant. CONCLUSION: Elevated extracellular glycine (Gly) content in bilirubin-toxic brain tissue can increase the sensitivity of NMDA receptor to Asp and Glu, which is the neurotransmitter of EAA, and cause over-activation of NMDA receptor