论文部分内容阅读
目的 探讨红藻氨酸 (KA,10 mg·kg- 1 )诱导大鼠癫痫发作时海马代谢型谷氨酸受体 5亚型 (m Glu R5 ) m RNA表达变化的病理特征 .方法 采用同位素 S35 d ATP标记寡核苷酸探针原位杂交法检测大鼠癫痫发作前后海马 m Glu R5m RNA的表达变化 ,通过图像分析系统进行定量处理 .同时观察大鼠行为学及脑电变化 .结果 KA注射后大鼠均出现严重边缘性惊厥 ,脑电图表现为阵发性癫痫样放电 .正常大鼠海马中有丰富的 m Glu R5 m RNA表达 ,以齿状回和 CA1区表达较高 .KA注射后海马各区 m Glu R5 m RNA表达呈时间依赖性下调 ,CA1,CA3,CA4区表达在 2 h呈现出显著性差异 (P<0 .0 5 ) ,齿状回区表达在 1h即有显著性差异 (P<0 .0 1) ,至 72 h各区表达均至最低 .结论 m Glu R5在癫痫发作后表达下降可能有助于限制神经元网络的异常兴奋性 ,使细胞发挥自身保护性作用
Objective To investigate the pathological changes of the expression of mGluR5 mRNA in the hippocampus of rats induced by kainic acid (KA, 10 mg · kg -1) induced by epilepsy in rats.Methods The isotope S35 d ATP-labeled oligonucleotide probe in situ hybridization detection of hippocampal mGlu R5m RNA expression changes in rats, quantitative analysis by image analysis system.At the same time observe the behavioral and EEG changes in rats.Results KA injection Severe fronto-seizures occurred in all the rats, and the EEG showed paroxysmal epileptiform discharges.In normal rats, the hippocampus was rich in mRNA expression of m Glu R5mRNA, which was highly expressed in the dentate gyrus and CA1 area. The expression of Glu R5 mRNA in the hippocampus was down-regulated in a time-dependent manner. The expression of CA1, CA3 and CA4 showed a significant difference at 2 hours (P <0.05). The expression of dentate gyrus in 1 h was significant (P <0.01), and reached the lowest in 72 h.Conclusion Decreasing expression of mGluR5 after seizure may help to limit the abnormal excitability of neuronal networks and make the cells play a protective role