不同寄主植物青枯菌菌株的比较研究

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根据番茄、茄、马铃薯、花生、甘薯、姜、桑、油橄榄、木麻黄九种植物上22个青枯菌的菌株,进行形态、染色、生理生化反应、致病性等方面的比较研究的结果,证明它们都是属于青枯假单胞菌(Pseudomonas solanacearumSmith)。因此,以前报道的甘薯瘟的病原细菌Pseudomonas batatas Tsengand Fan,应改为Pseudomonas solanacearum Sncith,不同寄主青枯菌菌株,它们的有些生理生化反应有明显的差异,但更为明显的是它们的致病性不同。测定的7种寄主上的青枯菌菌株,根据它们对茄的致病力很强,番茄、茄、姜、花生、甘薯、油橄榄和木麻黄菌株可归入小种1;马铃薯菌株对马铃薯的致病力很强,而对茄、番茄和辣椒的致病力中等,归入小种3。属于小种1的7种植物的菌株,致病性也有所不同,它们可作为不同的菌系。桑菌株的致病性较为特殊,很难归入已报道的小种。根据对6种糖和醇的利用以及脱氮作用的能力,番茄、茄、花生、油橄榄菌株属生物型Ⅱ;姜、木麻黄、甘薯菌株属生物型Ⅳ;马铃薯菌株属生物型Ⅱ,桑菌株属生物型Ⅰ。但桑菌株人工接种不侵染茄和马铃薯。烟叶过敏反应的测定表现黄斑而从未表现过敏反应,它的归属尚待进一步研究。青枯假单胞菌划分为小种和生物型,目前意见还不一致,加上我们测定的菌株有限,有关这方面的工作,也待进一步研究。此外,马铃薯、甘薯和桑菌株的生长适温是28℃,它们属于低温型菌株。这些低温型菌株的耐盐性较差。 Based on the results of comparative studies on morphology, staining, physiological and biochemical reactions and pathogenicity of 22 strains of R. solanacearum on nine species of tomato, tomato, potato, peanut, sweet potato, ginger, mulberry, olive and , Proving that they all belong to Pseudomonas solanacearum Smith. Therefore, the previously reported pathogenic bacteria Pseudomonas batatas Tsengand Fan of sweet potato blast should be changed to Pseudomonas solanacearum Sncith, different host strains of R. solanacearum, some of their physiological and biochemical reactions were significantly different, but more obvious is their pathogenic Different sex. According to their strong pathogenicity to tomato, the strains of R. solanacearum on the seven kinds of hosts were determined to be race 1, and the strains of tomato, eggplant, ginger, peanut, sweet potato, olive and wood ephedra could be classified as race 1; Very virulent, but moderate to tomato, pepper and capsicum, classified as race 3. Seven strains belonging to races 1 have different pathogenicity and may serve as different bacterial strains. Sang strains of the more specific pathogenicity, it is difficult to fall into the races have been reported. According to the utilization of six kinds of sugars and alcohols as well as the ability of denitrification, tomato, eggplant, peanut and olive tree belong to genotype Ⅱ; ginger, casuarina and sweet potato belong to genotype Ⅳ; potato strains belong to genotype Ⅱ and mulberry Is a biological type Ⅰ. However, artificial inoculation of Sang strain does not infect eggplant and potato. The determination of anaphylaxis in the tobacco leaf shows macular and never shows an allergic reaction, and its ownership remains to be further studied. Pseudomonas solanacearum is divided into races and biotypes, the current opinion is not consistent, coupled with the limited strains we measured, the work in this area, but also to be further studied. In addition, the optimum growth temperature for potato, sweet potato and mulberry strains is 28 ° C, which is a low temperature type strain. These low temperature strains have poor salt tolerance.
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