论文部分内容阅读
将携带有AtDREBlA基因,并以35S或rd29A启动子驱动的植物表达载体转入地被菊花(Dendranthema grandiflorum)的粉色品种‘Fall color’．与野生型相比,35S:DREBIA转基因植株表现出严重的生长抑制,而rd29A:DREB1A植株生长正常．RT-PCR检测表明,在胁迫条件下, AtDREB1A基因在35S:DREB1A转基因植株中呈现组成型过量表达,而在rd29A:DREB1A植株中则是受胁迫诱导型过量表达．这两种启动子驱动的转基因植株对干旱和盐溃胁迫都表现出较强的耐性,其中rd29A:DREB1A植株耐性显著强于35S:DREB1A植株．rd29A:DREB1A植株中的脯氨酸含量和SOD活性都强烈地被胁迫诱导升高,且高于35S:DREB1A植株．这些结果表明,在地被菊花中表达AtDREB1A基因可以提高植株对干旱和盐渍胁迫的耐性,同时这些耐性的升高可能与脯氨酸含量和SOD活性的上升有关．
The plant expression vector, carrying the AtDREB1A gene and driven by the 35S or rd29A promoter, was transferred to the pink variety ’Fall color’ of Dendranthema grandiflorum. The 35S: DREBIA transgenic plants showed severe growth inhibition compared to the wild type, while the rd29A: DREB1A plants grew normally. The results of RT-PCR showed that under stress conditions, AtDREB1A gene was constitutively overexpressed in 35S: DREB1A transgenic plants, while in rd29A: DREB1A plants, it was overexpressed by stress. Both of the two promoter-driven transgenic plants showed strong tolerance to drought and salt stress. Among them, the rd29A: DREB1A plants were significantly more resistant than the 35S: DREB1A plants. Proline content and SOD activity in rd29A: DREB1A plants were both strongly induced by stress and higher than that of 35S: DREB1A plants. These results indicate that the expression of AtDREB1A gene in the ground chrysanthemum can increase the tolerance of plants to drought and salinity stress, and the increase of these tolerance may be related to the increase of proline content and SOD activity.