论文部分内容阅读
根据日本血吸虫卵壳蛋白基因的已知序列，借助计算机核酸序列软件分析，设计了一对引物。在5’端引物中引入HindⅢ酶切位点和起始密码子，在3’端引物中引入EcoRⅠ酶切位点和终止密码子，用PCR方法对日本血吸虫虫卵、雌虫、雄虫的基因组DNA进行体外扩增。1％琼脂糖凝胶电泳显示：雌虫和虫卵的基因组DNAPCR产物在618bp处出现特异扩增条带，而雄虫及对照组则无此条带出现。该条带与预期长度相符，并对PCR产物进行纯化和酶切。
According to the known sequence of Schistosoma japonicum egg shell protein gene, a pair of primers was designed by computer software analysis of nucleic acid sequence. Hind Ⅲ restriction site and start codon were introduced into the 5 ’end primer, EcoR Ⅰ restriction site and stop codon were introduced into the 3’ end primer, and the PCR products were used to detect the DNA of Schistosoma japonicum eggs, females and males Genomic DNA was amplified in vitro. 1% agarose gel electrophoresis showed that genomic DNA of female and ovum genomic DNA showed a specific amplification band at 618bp, but no bands in male and control groups. The band matches the expected length and the PCR product is purified and digested.