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目的探讨邻苯二甲酸二丁酯(DBP)暴露诱发的小鼠肺部损伤。方法采用皮肤和经口摄入两种暴露方式染毒,在每一种暴露方式中,各选32只雄性Balb/C小鼠随机分为4组,每组8只。皮肤暴露的DBP剂量分别为0、4、40和400mg/kg,经口摄入(灌胃)的DBP剂量分别为0、0.4、4和40 mg/kg,染毒60 d后,对小鼠肺泡灌洗液中白细胞总数及分类进行计数,采用酶联免疫吸附(ELISA)法测定小鼠血清中免疫球蛋白E(IgE)的含量。同时,采用HE染色法观察小鼠肺部气道的病理学变化。最后,通过动物肺功能分析系统检测小鼠气道高反应性的变化。结果两种染毒方式的小鼠肺泡灌洗液中白细胞数、淋巴细胞、嗜酸性粒细胞和血清中IgE均随DBP染毒剂量的升高呈上升趋势,且与对照组相比,皮肤暴露组中DBP染毒剂量≥40 mg/kg,经口暴露组DBP染毒剂量≥4 mg/kg时,差异有统计学意义(P<0.05,P<0.01)。同时,在两种染毒方式中,随着DBP染毒剂量的升高,小鼠肺部气道出现明显病理学变化,但气道高反应性并无明显的改变。结论高剂量DBP暴露(皮肤≥40 mg/kg,经口≥4 mg/kg)可以提高小鼠肺泡灌洗液中白细胞、淋巴细胞、嗜酸性粒细胞和血清中IgE的水平,并且引起明显的气道病理学变化。
Objective To investigate the lung injury in mice induced by dibutyl phthalate (DBP) exposure. Methods Twenty-two male Balb / C mice were randomly divided into 4 groups with 8 mice in each group. DBP doses were 0, 4, 40, and 400 mg / kg, respectively. The dose of DBP was 0, 0.4, 4 and 40 mg / kg orally after oral administration. The total number and classification of leukocytes in alveolar lavage fluid were counted, and the content of immunoglobulin E (IgE) in the serum of the mice was determined by enzyme-linked immunosorbent assay (ELISA). At the same time, the pathological changes of lung airway in mice were observed by HE staining. Finally, the change of airway hyperresponsiveness in mice was detected by animal lung function analysis system. Results The numbers of leukocytes, lymphocytes, eosinophils and serum IgE in BALF were increased with the increase of DBP dose in mice exposed to both of the two methods. Compared with the control group, the skin exposed DBP dose ≥40 mg / kg, DBP dose ≥4 mg / kg in oral exposure group, the difference was statistically significant (P <0.05, P <0.01). At the same time, with the increase of DBP dose, the pathological changes in the lungs of mice showed significant changes in both modes of exposure, but there was no significant change in airway hyperresponsiveness. Conclusion Exposure to high doses of DBP (skin ≥40 mg / kg, oral ≥4 mg / kg) can increase the level of IgE in BALF, MIP and serum in mice Airway pathology changes.