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目的 探讨微囊化转酪氨酸羟化酶 (TH)基因的细胞在帕金森病 (PD)大鼠模型脑内存活、组织反应及对异常行为的治疗效果。 方法 以人 TH基因作为目的基因 ,重组到逆转录病毒载体感染人成纤维细胞 ,将细胞包裹在 APA半透膜中进行微囊化后 ,植入 6 -羟多巴胺单侧损毁的 PD大鼠模型纹状体内 ,观察移植物的存活状况和功能作用 16周。 结果 体外和植入体内的微囊化转基因细胞具有良好的存活能力。移植微囊化细胞可以使大鼠异常运动明显改善 ,移植位点周围的免疫反应较小。 结论 微囊化对转基因细胞异种移植以进行基因治疗具有显著意义
Objective To investigate the effects of microencapsulated tyrosine hydroxylase (TH) gene on survival, tissue response and abnormal behavior in Parkinson’s disease (PD) rat model. Methods Human TH gene was cloned into retrovirus vector to infect human fibroblasts. The cells were encapsulated in APA semipermeable membrane and then microencapsulated. The rats were implanted with 6 - hydroxydopamine unilateral damaged PD rat model In the striatum, the graft survival and function were observed for 16 weeks. Results Microencapsulated transgenic cells implanted in vitro and in vivo have good viability. Transplantation of microencapsulated cells can significantly improve the abnormal movement of rats, immune response around the transplantation site smaller. Conclusion Microencapsulation is of great significance for gene therapy of transgenic cell xenotransplantation