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目的 探讨荧光原位杂交 (FISH)微核作为生物剂量计及评价职业受照射人群辐射效应的可行性。方法 用1 37Csγ射线不同剂量 (0 1～ 2 5Gy)照射离体人外周血细胞 ,用泛着丝粒探针的FISH技术进行微核分析 ,拟合剂量效应方程 ;检测医用X射线工作者的无着丝粒微核率并估算其生物剂量。结果 离体照射条件下微核率随吸收剂量增加而增加 ,以无着丝粒微核增加为主 ,着丝粒微核仅有轻度增加。微核率与剂量之间的拟合曲线方程 ,CB微核法 :Y =0 0 0 5 +0 0 36D +0 0 11D2 ,R2 =0 99;FISH微核法 :Y =0 0 0 1+0 0 35D +0 0 0 7D2 ,R2 =0 99。结论 FISH微核法优于CB微核法。用FISH微核法对医用X射线工作者的检测结果显示无着丝粒微核率可以用来估算生物剂量。
Objective To investigate the feasibility of fluorescence in situ hybridization (FISH) micronuclei as a biological dosimeter and to evaluate the radiation effects of exposed workers. METHODS: Peripheral blood cells were irradiated with different doses of (1 1 ~ 2 5Gy) 1 37Csγ ray and micronuclei were analyzed by FISH technique with pan-centromere probes to fit the dose-response equation. The detection of no medical X-ray workers Centromere micronucleus rate and estimate its biological dose. Results The rate of micronucleus increased with the increase of absorbed dose under the condition of in vitro irradiation, with the increase of non-centromere micronucleus and only slight increase of centromere micronucleus. The fitted curve equation between micronucleus rate and dose, CB micronucleus method: Y = 0 0 0 5 +0 0 36D +0 0 11D2, R2 = 0 99; FISH micronucleus method: Y = 0 0 0 1+ 0 0 35D +0 0 0 7D2, R2 = 0 99. Conclusion FISH micronucleus method is superior to CB micronucleus method. The results of FISH micronucleus tests on medical X-ray workers show that the absence of centromeric micronuclei can be used to estimate biological dosages.