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目的观察肿瘤坏死因子相关凋亡诱导配体(TRAIL)对裸鼠胃癌移植瘤的生长抑制作用,探讨死亡相关蛋白3(DAP3)与其的关系。方法建立人胃癌细胞株BGC-823裸鼠移植瘤模型；观察不同剂量的TRAIL(0、1、5、10mg/kg体重)对移植瘤的生长抑制作用；流式细胞仪测定细胞周期及凋亡情况；Western blot和逆转录-聚合酶链反应(RT-PCR)检测DAP3蛋白和DAP3 mR- NA表达情况。结果5 mg/kg体重和10 mg/kg体重两组的瘤重分别为(2．57±0．35)g和(2．49±0．26)g,均低于1 mg/kg体重组(3．23±0．32)g和对照组(3．57±0．47)g,前两组的细胞增殖指数分别为(36．69±5．16)%和(39．69±6．13)%,低于对照组(48 06±6．99)%；Western blot和RT- PCR法分别测得前两组中DAP3蛋白和mRNA的表达高于1 mg/kg体重组和对照组。结论TRAIL可有效抑制裸鼠胃癌移植瘤的生长,其作用机制可能与诱导DAP3表达有关。
Objective To observe the growth inhibitory effect of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) on gastric cancer xenografts in nude mice and to explore the relationship between death associated protein 3 (DAP3) and it. Methods The human gastric cancer cell line BGC-823 xenografted model was established. The growth inhibitory effect of different doses of TRAIL (0, 1, 5 and 10 mg / kg body weight) on the xenografts was observed. The cell cycle and apoptosis were determined by flow cytometry Western blot and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect the expression of DAP3 protein and DAP3 mRNA. Results The tumor weights in the 5 mg / kg body weight and 10 mg / kg body weight groups were (2.57 ± 0.35) g and (2.49 ± 0.26) g respectively, both lower than 1 mg / kg body weight (3.23 ± 0.32) g and control group (3.57 ± 0.47) g respectively. The proliferation index of the two groups were (36.69 ± 5.16)% and (39.69 ± 6) .13%), lower than the control group (48 06 ± 6.99)%; Western blot and RT-PCR measured the expression of DAP3 protein and mRNA in the first two groups were higher than the 1 mg / kg body weight and the control group . Conclusion TRAIL can effectively inhibit the growth of transplanted gastric cancer in nude mice. The mechanism may be related to the induction of DAP3 expression.