Qinghai Province is an important component of the Qinghai-Tibet Plateau in China. Scientific evaluation of the suitability of Qinghai’s climate for tourism can contribute to overcoming obstacles posed by climate on sustainable tourism development in Qinghai Province, including disparities between the low and high seasons, high altitude health concerns, and weather events. A tourism climate suitability evaluation model of the Qinghai-Tibet Plateau is constructed (Tourism Climate Suitability Index, or TCSI), and tourism climate suitability is comprehensively evaluated for Qinghai Province from climate data from 1960 to 2009. Results show that: (I) There is clear distributional characteristics of spatial-temporal variability of TCSI values in Qinghai Province. (II) Tourism climate suitability in Qinghai Province has significant seasonal and regional differences. The year is divided into a very suitable period (July and August), suitable tourism periods (from April and October), less suitable periods (From Nov to Mar). June to August is the most suitable tourism period in Qinghai. Qinghai Province is divided into five levels of tourism climate suitability: most suitable regions, very suitable regions, suitable regions, less suitable regions, and unsuitable region. (III) The key factor which influences regional differences in tourism climatic suitability is atmospheric oxygen. And the key factors which chiefly influence seasonal differences of tourism climate suitability are temperature and humidity, the wind chill factor, and barrier weather. Scientific evaluation of the suitability of Qinghai’s climate for tourism can contribute to overcoming obstacles posed by climate on sustainable tourism development in Qinghai Province, including disparities between the low and high seasons, high altitude health concerns, and weather events. A tourism climate suitability evaluation model of the Qinghai-Tibet Plateau is constructed (Tourism Climate Suitability Index, or TCSI), and tourism climate suitability is comprehensively evaluated for Qinghai Province from climate data from 1960 to 2009 . Results show that: (I) There is clear distributional characteristics of spatial-temporal variability of TCSI values ​​in Qinghai Province. (II) Tourism climate suitability in Qinghai Province has significant seasonal and regional differences. The year is divided into a very suitable period (July and August), suitable tourism periods (from April and October), less suita bleaching (From Nov to Mar). June to August is the most suitable tourism period in Qinghai. Qinghai Province is divided into five levels of tourism climate suitability: most suitable regions, very suitable regions, suitable regions, less suitable regions, and unsuitable (III) The key factor which influences regional differences in tourism climatic suitability is atmospheric oxygen. And the key factors which chiefly influence seasonal differences of tourism climate suitability are temperature and humidity, the wind chill factor, and barrier weather.