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将聚甲基丙烯酸甲酯材料在多壁碳纳米管表面原位聚合,利用这种修饰的碳纳米管作为基质辅助激光解析离子化(MALDI)的基质,利用修饰后的碳纳米管可以“溶解”的特性实现了稳定的MALDI离子化,并且消除了在低质量端的基质噪音.此类聚合物衍生的碳纳米管具有相对较好的亲水性表面,可“溶解”在溶剂中.此方法适用于有机小分子、多肽、聚合物和蛋白质酶解肽段的质谱分析.实验表明聚甲基丙烯酸甲酯固定化碳纳米管能有效地吸收和传递激光能量,可与样品充分地分散混合,质谱检测背景低,重现性好,具有较宽的可测质量范围.此方法在小分子快速检测和蛋白质组学方面有很大的应用前景.
The polymethylmethacrylate material is polymerized in situ on the surface of the multi-walled carbon nanotubes. By using the modified carbon nanotubes as a substrate for the matrix-assisted laser desalting (MALDI), the modified carbon nanotubes can be polymerized by using “ Dissolved ”characterizes stable MALDI ionization and eliminates matrix noise at the lower end of the spectrum. Such polymer-derived carbon nanotubes have relatively good hydrophilic surfaces that can be“ dissolved ”in solvents .The method is suitable for the mass spectrometry analysis of small peptides, peptides, polymers and proteolysis peptides.The experiments show that polymethylmethacrylate immobilized carbon nanotubes can effectively absorb and transfer laser energy, Disperse and mix, the detection background of the mass spectrometry is low, the reproducibility is good, has a wide measurable quality range.This method has great application prospect in small molecule rapid detection and proteomics.