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本文根据恶性疟原虫ＭＳＰ１１９已知序列，自行设计一对用于特异扩增ＭＳＰ１Ｃ端１９肽基因的引物Ｐ１、Ｐ２，在Ｐ１引物中引入ＳａｌⅠ酶切位点及ＡＴＧ起始密码子，在Ｐ２引物中引入ＸｂａⅠ酶切位点及终止密码子，经ＰＣＲ扩增获得３６３ｂｐ大小片段，与预期大小相符。采用“压碎与浸泡法”纯化回收ＰＣＲ产物，ＰＣＲ产物经套式ＰＣＲ及酶切鉴定证实与预期大小相符，说明所获确为ＭＳＰ１１９基因。为进一步将该基因与ＰｆＣＭＲ基因进行重组表达复合抗原和免疫接种奠定基础。
Based on the known sequence of Plasmodium falciparum MSP119, we designed a pair of primers P1 and P2 for specific amplification of the MSP1 C-terminal 19 peptide gene, introduced Sal Ⅰ restriction site and ATG start codon into P1 primer, Introduced Xba Ⅰ restriction sites and stop codons, amplified by PCR 363bp size fragment, in line with the expected size. The PCR products were purified by crushing and soaking method. The PCR products were verified by nested PCR and restriction enzyme digestion, which was consistent with the expected size, indicating that the MSP119 gene was obtained. It laid the foundation for the further recombination of this gene and PfCMR gene for the recombinant antigen and immunization.