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利用实验室制备的一种生物诱抗剂(Biological resistance inducer,BRI)成功诱导玉米对圆斑病菌(Cochliobolus carbonum)的抗性,BRI对玉米圆斑病菌孢子萌发及菌丝生长无明显影响。温室接种试验结果表明,BRI浓度在10～100μg/mL范围内,随着BRI浓度提高,其诱导玉米抗圆斑病的效果也相应提高。当BRI处理玉米后1 h再接种玉米圆斑病菌,能诱导玉米产生抗性、减轻病害;随着病菌接种时间的推移,其诱导抗性逐渐加强,控病效果逐渐提高;在接种前5 h喷施BRI,其防病效果达峰值(77.46%);接种前6 h喷施BRI,防病效果缓慢下降。当接种孢子在1×103～5×104个/mL时,BRI防病效果达60.95%～71.46%;当接种孢子达1×105个/mL时,防病效果仅达18.08%。
The resistance of corn to Cochliobolus carbonum was induced successfully by a laboratory-established biological resistance inducer (BRI). BRI had no significant effect on the spore germination and mycelial growth of C. turcica. The results of greenhouse inoculation showed that with BRI concentration increasing from 10 μg / mL to 100 μg / mL, the effect of BRI on the resistance to blooming of maize also increased accordingly. Inoculation of A. punctatus 1 h after treatment with BRI could induce the resistance of maize and alleviate the disease. With the passage of time, the induced resistance gradually increased and the disease control effect gradually increased. In the 5 h before inoculation The effect of preventing disease was peaked by spraying BRI (77.46%). BRI was applied 6 h before inoculation, and the disease control effect decreased slowly. When inoculated sporulation in 1 × 103 ~ 5 × 104 个 / mL, BRI disease prevention effect of 60.95% ~ 71.46%; when inoculated spores up to 1 × 105 个 / mL, the disease control effect of up to 18.08%.