论文部分内容阅读
目的探讨CXCR4 shRNA对胶质瘤细胞U251体外增殖和侵袭能力的影响,为CXCR4 shRNA治疗胶质瘤提供实验依据。方法用脂质体Lipofectimine TM2000将CXCR4-shRNA转染U251细胞,MTT实验检测转染细胞的增殖,Transwell侵袭实验检测转染细胞的迁移和侵袭能力。结果与转染非特异的pGPU6/GFP/NC组和未转染组比较,转染CXCR4-shRNA载体pGPU6/GFP/Rac1-421组的U251细胞CXCR4 mRNA显著降低(P<0.05),并且细胞体外增殖能力减弱(P<0.05),侵袭到滤膜底面的细胞数为36.67±9.76,分别低于对应的pGPU6/GFP/NC组和未转染组(P<0.05)。结论 CXCR4 shRNA载体pGPU6/GFP/Rac1-421可下调U251细胞CXCR4的表达,抑制U251细胞的增殖和迁移侵袭能力,提示CXCR4在胶质瘤发展过程中具有重要作用。
Objective To investigate the effect of CXCR4 shRNA on proliferation and invasion of glioma U251 in vitro and to provide experimental evidence for CXCR4 shRNA treatment of glioma. Methods CXCR4-shRNA was transfected into U251 cells with Lipofectimine TM2000. MTT assay was used to detect the proliferation of transfected cells. Transwell invasion assay was used to detect the migration and invasion ability of transfected cells. Results The CXCR4 mRNA of U251 cells transfected with CXCR4-shRNA vector pGPU6 / GFP / Rac1-421 was significantly lower (P <0.05) compared with non-specific pGPU6 / GFP / NC transfected cells and untransfected cells (P <0.05). The number of cells that infiltrated the bottom of the filter membrane was 36.67 ± 9.76, which was lower than that of the corresponding pGPU6 / GFP / NC group and the untransfected group (P <0.05). Conclusion CXCR4 shRNA vector pGPU6 / GFP / Rac1-421 can down-regulate the CXCR4 expression in U251 cells and inhibit the proliferation and migration of U251 cells, suggesting that CXCR4 plays an important role in the development of glioma.