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目的观察模拟失重不同时期大鼠比目鱼肌肌梭超微结构的改变。方法采用大鼠尾部悬吊法建立模拟失重模型。雌性大鼠随机分为模拟失重 4、7、1 4d组和模拟失重 1 4d后恢复 1 4d组 ,以及各组的对照组。分离单一肌梭 ,用透射电镜观察模拟失重不同时期比目鱼肌肌梭超微结构的改变。结果模拟失重 4d组大鼠比目鱼肌肌梭的超微结构有较明显的改变 ,表现为部分肌原纤维排列略见紊乱 ,线粒体增生、肿胀 ,终末池明显扩张等。模拟失重 7d组肌梭的超微结构改变更为明显。模拟失重 1 4d组肌梭超微结构改变显著 ,可见严重的退行性改变 ,超微结构模糊不清 ,细胞核固缩 ,线粒体增生、肿胀 ,线粒体嵴模糊、减少、消失 ,Z线排列紊乱 ,部分溶解消失 ,肌浆网终末池高度扩张。模拟失重 1 4d后恢复 1 4d组肌梭超微结构基本恢复正常。结论模拟失重可致肌梭超微结构发生明显的、可逆性的改变 ,随模拟失重时间延长而加重
Objective To observe the ultrastructural changes of the muscle spindles in different periods of simulated weightlessness in rats. Methods The rat tail suspension method was used to establish simulated weightlessness model. Female rats were randomly divided into simulated weightless 4,7,1 4d group and 14 days after simulated weight loss recovered 14d group, and the control group of each group. The single muscle spindle was isolated and the ultrastructural changes of the muscle spindles were simulated by transmission electron microscopy. Results The ultrastructural changes of the muscle spindles in the simulated soleus 4d group showed obvious changes, which showed that the arrangement of some myofibrils was slightly disordered, mitochondria hyperplasia and swelling, and the terminal pool was obviously dilated. The ultrastructural changes of muscle spindle in simulated weightless 7d group were more obvious. The ultrastructural changes of the muscle spindle in the 14th day after simulated weightlessness were significant, with severe degenerative changes, unclear ultrastructure, nuclear condensation, mitochondrial hyperplasia, swelling and mitochondrial cristae vaguely diminished and disappeared. Dissolved disappear, sarcoplasmic reticulum terminal height expansion. After 4 days of simulated weightlessness recovery, the ultrastructure of muscle spindle returned to normal after 14 days. Conclusion Simulated weightlessness can lead to obvious and reversible changes in the ultrastructure of muscle spindle, which aggravate with the prolongation of simulated weightlessness