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目的探讨某地区东方立克次体流行株的基因型和遗传特征。方法采用荧光定量PCR技术对该地区东方立克次体感染患者和鼠类标本Sta56蛋白编码基因片段检测,对阳性标本进行基因分型和测序,并与巢式PCR分型结果相比较;对测序结果与GenBank东方立克次体参考序列进行同源性分析。结果东方立克次体感染患者和鼠类标本中可扩增出Gilliam型523bp目的基因片段,与Kawasaki型相似,同源性为96.3%;鼠类标本中还可扩增出318bp目的基因片段,与TA686型相似,同源性为78.5%。东方立克次体感染患者外周血中东方立克次体核苷酸序列与鼠标本的同源性为100.0%。结论粤北山区东方立克次体至少存在Gilliam型和Kawasaki型相似株,鼠类中存在TA686型相似株。粤北山区人、鼠均存在东方立克次体自然感染,已成为恙虫病自然疫源地。
Objective To investigate the genotype and genetic characteristics of Rickettsia orientalis in a certain area. Methods Fluorescent quantitative PCR was used to detect the gene fragment encoding Sta56 protein in Rickettsia orientalis and rodent specimens of the Oriental region. The positive samples were genotyped and sequenced and compared with the results of nested PCR. Results The homology analysis was carried out with the reference sequence of Rickettsia orientalis in GenBank. Results The 521bp Gilliam gene fragment was amplified from Rickettsia orientalis and murine samples, which was similar to Kawasaki type with a homology of 96.3%. A 318bp gene fragment was amplified in murine samples, Similar to TA686 type, the homology is 78.5%. The identities of Rickettsia orientalis and mouse in the peripheral blood of patients with Rickettsia orientalis were 100.0%. Conclusion There are at least Gilliam-type and Kawasaki-type strains in Rickettsia orientalis in the mountainous area of northern Guangdong, and TA686-like strains exist in the rodents. Northern mountainous people, rats are present in the natural infection of Rickettsia orientalis has become a natural foci of scrub typhus.