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目的探讨海鞘醇类似物002抗肿瘤活性。方法采用MTS法检测不同浓度的海鞘醇类似物对HepG2、A549和NIH3T3等细胞增殖的抑制作用;流式细胞、Annexin V-FITS/7-AAD等技术检测海鞘醇类似物对HepG2、A549和NIH3T3等细胞凋亡的影响。结果海鞘醇类似物对HepG2和A549的增殖有明显抑制作用,抑制率分别为73.67%和62.4%;海鞘醇类似物在浓度为2.0μg/ml时,引起HepG2和A549细胞的早期凋亡率分别为25.72%和25.46%。而对NIH3T3细胞影响不大。结论海鞘醇类似物对人肝癌和肺癌细胞的增殖有较强抑制作用,且能促进人肝癌和肺癌细胞的早期凋亡。
Objective To investigate the antitumor activity of the analogue 002 of Methods MTS was used to detect the inhibitory effect of different concentrations of ascidol on proliferation of HepG2, A549 and NIH3T3 cells. Flow cytometry and Annexin V-FITS / 7-AAD were used to detect the effects of the analogs of HepG2, A549 and NIH3T3 Such as the impact of apoptosis. Results The results showed that the proliferation of HepG2 and A549 was significantly inhibited by the analogues of SD. The rates of inhibition were 73.67% and 62.4%, respectively. While at the concentration of 2.0 μg / ml, the early apoptosis rates of HepG2 and A549 25.72% and 25.46%. While little impact on NIH3T3 cells. Conclusion The analogues of Sarcocapsules strongly inhibit the proliferation of human hepatocarcinoma and lung cancer cells, and promote the early apoptosis of human hepatocarcinoma and lung cancer cells.