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以莱牧1号幼苗为实验材料,在不同浓度PEG-6000干旱胁迫条件下,测定幼苗叶片的叶绿素含量、叶片相对含水量、渗透液相对电导率、丙二醛含量、脯氨酸含量、可溶性多糖含量、可溶性蛋白含量的变化,并通过灰色关联度分析各指标与莱牧1号抗旱性的关系。结果表明:随着PEG-6000浓度的提高,叶绿素、叶片相对含水量、相对电导率、可溶性蛋白含量下降,与胁迫质量浓度呈显著负相关;可溶性多糖含量升高,丙二醛含量呈先增加后降低的趋势,脯氨酸含量先降低后升高,且三项指标变化均与胁迫质量浓度呈显著正相关。各生理指标与抗旱指数的关联度依次为:叶片相对含水量(0.980 8)、叶绿素含量(0.967 9)、丙二醛含量(0.876 0)、可溶性多糖含量(0.839 5)、可溶性蛋白含量(0.827 5)、叶片渗透液相对电导率(0.670 8)、脯氨酸含量(0.571 3)。
Seedlings of Laimu 1 were used as experimental materials under different concentrations of PEG-6000 drought stress to determine the leaf chlorophyll content, relative leaf water content, relative conductivity of permeate, malondialdehyde content, proline content, soluble Polysaccharide content and soluble protein content, and analyzed the relationship between the index and drought resistance of Limei 1 by gray relational analysis. The results showed that with the increase of PEG-6000 concentration, the relative water content, relative conductivity and soluble protein content of chlorophyll and leaf decreased significantly, but negatively correlated with stress mass concentration. The content of soluble polysaccharide increased and the content of malondialdehyde increased first After reducing, the content of proline decreased first and then increased, and the changes of three indexes were positively correlated with stress mass concentration. The correlations between drought resistance index and physiological index were as follows: leaf relative water content (0.980 8), chlorophyll content (0.967 9), malondialdehyde content (0.876 0), soluble polysaccharide content (0.839 5), soluble protein content 5), relative conductivity (0.670 8) and proline content (0.571 3) of leaf permeate.