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目的:研究地西他滨单独或联合5-氟尿嘧啶/紫杉醇对胃癌SGC-7901细胞株的抑制作用。方法:应用MTS方法检测地西他滨单独或联合5-氟尿嘧啶/紫杉醇作用于胃癌SGC-7901细胞株后,在不同浓度梯度和不同时间在490nm波长处的吸光度(A)值,并计算细胞增殖抑制率。同时,采用流式细胞仪检测中间浓度组各药物单独或联合应用在48h对细胞凋亡的影响。MTS检测时间分别选择细胞增殖12、24、36、48、60及72h,流式细胞仪检测时间选择48h。结果:地西他滨对胃癌SGC-7901细胞的增殖抑制作用均随着时间和浓度的递增而逐渐升高;地西他滨联合5-氟尿嘧啶或紫杉醇组对胃癌细胞的抑制率均较单药组升高(P<0.05)。在诱导细胞凋亡方面,地西他滨联合用药组48h的细胞凋亡率均高于单独用药组(P<0.05)。结论:地西他滨能增强5-氟尿嘧啶和紫杉醇对胃癌SGC-7901细胞体外增殖的抑制作用,能促进5-氟尿嘧啶和紫杉醇对胃癌SGC-7901细胞的凋亡。
Objective: To study the inhibitory effect of decitabine alone or in combination with 5-fluorouracil / paclitaxel on gastric cancer cell line SGC-7901. Methods: The MTS assay was used to detect the absorbance (A) of decitabine alone or in combination with 5-fluorouracil / paclitaxel in gastric cancer cell line SGC-7901 at 490nm wavelength with different concentration gradient and different time, and the cell proliferation Inhibition rate. At the same time, using flow cytometry to detect the concentration of each group of drugs alone or in combination at 48h on apoptosis. MTS detection time were selected cell proliferation 12,24,36,48,60 and 72h, flow cytometry selection time 48h. Results: Decitabine inhibited the proliferation of gastric cancer SGC-7901 cells gradually with the increasing of time and concentration. The inhibitory rate of decitabine combined with 5-fluorouracil or paclitaxel group was higher than that of single drug Group increased (P <0.05). In the induction of apoptosis, the apoptotic rate of the combination group of decitabine 48h was higher than that of the drug alone group (P <0.05). CONCLUSION: Decitabine can enhance the inhibitory effect of 5-fluorouracil and paclitaxel on the proliferation of gastric cancer cell SGC-7901 in vitro and promote the apoptosis of gastric cancer cell SGC-7901 by 5-fluorouracil and paclitaxel.