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目的:鉴定短肽GMBP42与胃癌多药耐药细胞(SGC7901/VCR和SGC7901/ADR)的结合特异性及其多药耐药逆转活性。方法:体外培养胃癌亲本细胞SGC7901及多药耐药细胞(SGC7901/VCR和SGC7901/ADR)。免疫细胞化学染色技术鉴定短肽GMBP42与多药耐药细胞的结合特异性。体外药物敏感实验测定短肽GMBP42对多药耐药细胞化疗药物敏感性的影响。结果:免疫细胞化学染色结果显示:短肽GMBP42能够与胃癌多药耐药细胞(SGC7901/VCR和SGC7901/ADR)结合,亚细胞定位于核周胞浆及胞膜,而与亲本细胞无明显结合。体外药物敏感试验结果显示:与对照组相比,短肽GMBP42处理组胃癌多药耐药细胞(SGC7901/VCR和SGC7901/ADR)对化疗药物的IC50值及细胞存活率均降低(P<0.05)。结论:短肽GMBP42能特异结合于多种胃癌多药耐药细胞,短肽GMBP42可提高胃癌多药耐药细胞对阿霉素的敏感性,部分逆转多药耐药细胞对阿霉素的耐药表型。
Objective: To identify the binding specificity of GMBP42 and multidrug resistance reversal activity of multidrug resistant gastric cancer cells (SGC7901 / VCR and SGC7901 / ADR). Methods: Gastric cancer parental cells SGC7901 and multidrug resistance cells (SGC7901 / VCR and SGC7901 / ADR) were cultured in vitro. Immunocytochemical staining technique was used to identify the binding specificity of short peptide GMBP42 to multidrug resistant cells. In vitro drug sensitivity assay to determine the effect of peptide GMBP42 on chemosensitivity of multidrug resistant cells. Results: The results of immunocytochemical staining showed that the short peptide GMBP42 could bind to multidrug-resistant cells (SGC7901 / VCR and SGC7901 / ADR) in gastric cancer. The subcellular localization of peritoneal macrophages in the perinuclear cytoplasm and cell membrane, but no obvious binding with the parental cells . In vitro drug sensitivity test results showed that IC50 values and cell viability of chemotherapeutic agents were decreased in SGC7901 / VCR and SGC7901 / ADR cells treated with short peptide GMBP42 compared with the control group (P <0.05) . CONCLUSION: The short peptide GMBP42 can bind specifically to many multidrug resistant gastric cancer cells. The short peptide GMBP42 can enhance the sensitivity of multidrug resistant gastric cancer cells to doxorubicin, and partially reverse the resistance of multidrug resistant cells to doxorubicin Drug phenotype.