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Objective:To investigate the effects of E7080 and N5-(1-iminoethyl)-L-ornithine dihydrochloride(L-NIO)on colorectal cancer alone and in combination.Methods:HT29 colorectal cancer cell line from Sap Institute was used.Real-time cell analysis(xCELLigence system)was performed to determine the effects of E7080 and L-NIO on colorectal cell proliferation.While apoptosis was determined with Annexin V staining,and the effect of agents on angiogenesis was determined with chorioallantoic membrane(CAM)model.Results:We found that E7080 has a strong antiproliferative effect with an half maximum inhibition of concentration(IC50)value of 5.60×10–8mol/L.Also it has been observed that E7080 showed antiangiogenic and apoptotic effects on HT29 colorectal cancer cells.Antiangiogenic scores of E7080 were 1.2,1.0 and 0.6for 100,10 and 1 nmol/L E7080 concentrations,respectively.Furthermore,apoptosis has been detected in71%of HT29 colorectal cancer cells after administration of 100 nmol/L E7080 which may indicate strong apoptotic effect.Meanwhile administration of L-NIO alone did not show any effect,but the combination of E7080 with L-NIO increased the antiproliferative,antiangiogenic and apoptotic effects of E7080.Conclusions:Results of this study indicate that E7080 may be a good choice in treatment of colorectal tumors.Furthermore the increased effects of E7080 when combined with L-NIO raise the possibility to use a lower dose of E7080 and therefore avoid/minimize the side effects observed with E7080.
Objective: To investigate the effects of E7080 and N5- (1-iminoethyl) -L-ornithine dihydrochloride (L-NIO) on colorectal cancer alone and in combination. Methods: HT29 colorectal cancer cell line from Sap Institute was used. Real-time Cell analysis (xCELLigence system) was performed to determine the effects of E7080 and L-NIO on colorectal cell proliferation. Whiver apoptosis was determined with Annexin V staining, and the effect of agents on angiogenesis was determined with chorio allantoic membrane (CAM) model. Results : We found that E7080 has a strong antiproliferative effect with an half maximum inhibition of concentration (IC50) value of 5.60 × 10 -8 mol / L. Al so it has been observed that E7080 showed antiangiogenic and apoptotic effects on HT29 colorectal cancer cells. Antiangiogenic scores of E7080 were 1.2, 1.0 and 0.6 for 100, 10 and 1 nmol / L E7080 concentrations, respectively. Still further, apoptosis has been detected in 71% of HT29 colorectal cancer cells after administration of 100 nmol / L E7080 which may i ndicate strong apoptotic effect. Meanwhile while administration of L-NIO alone did not show any effect, but the combination of E7080 with L-NIO increased the antiproliferative, antiangiogenic and apoptotic effects of E7080. Conlusions: Results of this study that that E7080 may be a good choice in treatment of colorectal tumors. Frthermore the increased effects of E7080 when combined with L-NIO raise the possibility to use a lower dose of E7080 and therefore avoid / minimize the side effects observed with E7080.