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[目的]观察百草枯(PQ)对体外培养人胚肺成纤维细胞(MRC-5)的毒性作用,并探讨可能的损伤机制。[方法]不同浓度(0.00、0.15、0.30、0.60、1.20、2.40、4.80、9.60mmol/L)PQ处理MRC-5细胞24h后,采用四甲基偶氮唑盐微量酶反应比色法(MTT法)测定MRC-5细胞存活率,流式细胞术检测细胞凋亡情况;同时用分光光度比色法测不同浓度(0.00、0.15、0.30、0.60、1.20mmol/L)PQ处理3、12、24h后,MRC-5细胞超氧化物歧化酶(SOD)、丙二醛(MDA)含量和细胞上清液中乳酸脱氢酶(LDH)活性。[结果]与对照组相比,PQ≥0.30mmol/L时开始明显抑制细胞生长,并随着染毒浓度的增加细胞存活率明显下降(P<0.05)。与对照组相比,短时间、低剂量(3、12h,0.15mmol/L)作用下,SOD活性下降不明显,其他组别明显下降(P<0.05);与对照组相比,染毒后不同时点MDA含量均较自身对照上升,LDH含量较对照上升(除染毒3h,1.20mmol/L组),且差异有统计学意义(P<0.05或P<0.01);0.60、1.20mmol/L组PQ染毒后MRC-5细胞凋亡率明显上升(P<0.05)。[结论]PQ对MRC-5细胞活性的影响存在剂量依赖性,并可影响MRC-5细胞的抗氧化酶活性和细胞凋亡。
[Objective] To observe the toxic effects of paraquat (PQ) on cultured human embryo lung fibroblasts (MRC-5) and to explore the possible mechanism of injury. [Methods] MRC-5 cells were treated with different concentrations of PQ for 24 h, and the MTT assay was used to detect MTT Method) to determine the survival rate of MRC-5 cells, flow cytometry detection of apoptosis; at the same time using spectrophotometric colorimetric determination of different concentrations (0.00,0.15,0.30,0.60,1.20mmol / L) PQ treatment 3,12, The content of superoxide dismutase (SOD) and malondialdehyde (MDA) in MRC-5 cells and lactate dehydrogenase (LDH) activity in the supernatant of MRC-5 cells were detected after 24 hours. [Results] Compared with the control group, PQ≥0.30mmol / L began to significantly inhibit cell growth, and with the increase of exposure concentration, cell viability decreased significantly (P <0.05). Compared with the control group, the SOD activity did not decrease obviously in short time and low dose (3,12h, 0.15mmol / L), but decreased in other groups (P <0.05). Compared with the control group, (P <0.05 or P <0.01); 0.60 and 1.20 mmol / L of MDA at different time points increased compared with self-control, The apoptotic rate of MRC-5 cells in L group was significantly increased after PQ exposure (P <0.05). [Conclusion] The effects of PQ on the activity of MRC-5 cells in a dose-dependent manner, and can affect the antioxidant enzyme activity and apoptosis of MRC-5 cells.