论文部分内容阅读
目的:探讨秋海棠不同溶剂提取物的抗炎作用及其机制研究。方法:制备秋海棠醇提取物、石油醚提取物、乙酸乙酯提取物、正丁醇提取物、水提取物,以脂多糖(LPS)诱导RAW 264.7巨噬细胞活化,不同溶剂提取物作用24 h后,ELISA法检测上清液中肿瘤坏死因子-α(TNF-α)、白细胞介素1β(IL-1β)的含量,Griess法测定一氧化氮(NO)的含量。结果:秋海棠乙酸乙酯提取物、正丁醇提取物能极显著抑制TNF-α、IL-1β、NO的含量(P<0.01),秋海棠醇提取物、乙酸乙酯提取物能极显著抑制NO的含量(P<0.01)。结论:秋海棠醇提取物、乙酸乙酯提取物、正丁醇提取物抗炎作用显著,其机制可能与减少炎症因子的释放有关。
Objective: To investigate the anti-inflammatory effect of different solvent extracts of begonia and its mechanism. METHODS: Begonia extract, petroleum ether extract, ethyl acetate extract, n-butanol extract and water extract were prepared. Lipopolysaccharide (LPS) -induced RAW 264.7 macrophages were activated with different solvent extracts for 24 h The contents of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the supernatant were detected by ELISA. The content of nitric oxide (NO) was determined by Griess method. Results: Ethyl acetate extract and n-butanol extracts of Begonia could significantly inhibit the content of TNF-α, IL-1β and NO (P <0.01). The extract of Begonia and the ethyl acetate extract could significantly inhibit NO (P <0.01). CONCLUSION: The anti-inflammatory effects of alcohol extract of Begonia, ethyl acetate extract and n-butanol extract are significant, which may be related to the reduction of the release of inflammatory cytokines.