Whitmania pigra has been used as a traditional Chinese medicine (TCM) for promoting blood circulation,alleviating blood coagulation,activating meridians and relieving stasis for several hundred years.However,the therapeutic components of this species,especially proteins and peptides were poorly exploited.Until now only a few of them were obtained by using chromatographic isolation and purification.In recent decade,transcriptome techniques were rapidly developed,and have been used to fully reveal the functional components of many animal venoms.In the present study,the cDNA of the salivary gland of Whitmania pigra was sequenced by illumina and the transcriptome was assembled by using Trinity.The proteome were analysed by LC-MS/MS.Based on the data of the transcriptome and the proteome,a potential antiplatelet protein named pigrin was found.Pigrin was cloned and expressed using P.pastoris GS 115.The antiplatelet and antithrombotic bioactivities of pigrin were tested by using aggregometer and the rat arterio-venous shunt thrombosis model,respectively.The bleeding time of pigrin was measured by a mice tail cutting method.The docking ofpigrin and protease-activated receptor 1 (PAR1) or collagen were conducted using the ZDOCK Server.Pigrin was able to selectively inhibit platelet aggregation stimulated by PAR1 agonist and collagen.Pigrin attenuated thrombotic formation in vivo in rat,while did not prolong bleeding time at its effective dosage.There are significant differences in the key residues participating in binding of Pigrin-Collagen complex from Pigrin-PAR1 complex.In conclusion,a novel PAR1 inhibitor pigrin was found from the leech Whitmania pigra.This study helped to elucidate the mechanism of the leech for the treatment of cardiovascular disorder.