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连作障碍问题严重制约人参生产及土地资源的合理利用,自毒作用是导致连作障碍的关键因子之一。在前期筛选到人参自毒物质降解菌的基础上,研究采用平板培养法和常规生理生化方法测定人参种子萌发的生物学指标和保护性酶活性,以探究自毒物质降解菌对人参自毒作用的缓解效果。研究发现,除棕榈酸外,自毒物质苯甲酸、邻苯二甲酸二异丁酯、丁二酸二异丁酯和2,2-(4-羟基苯基)丙烷降解液对人参种子生长的毒害作用得到显著缓解,与对照无显著差异。除苯甲酸外,其余自毒物质降解后人参SOD,POD,CAT酶活性均较降解前显著降低。研究结果表明,微生物降解是缓解自毒物质抑制人参种子萌发的有效途径,该发现将有助于人参连作障碍问题的解决。
The problem of continuous cropping obstacle seriously restricts the rational utilization of ginseng production and land resources. The autotoxic effect is one of the key factors that cause obstacle in continuous cropping. On the basis of the screening of ginsenosides-degrading bacteria, the biological indicators and protective enzyme activities of ginseng seeds were determined by plate culture method and conventional physiological and biochemical methods in order to explore the effect of self-toxic material degrading bacteria on ginseng autotoxicity Ease the effect. The study found that, in addition to palmitic acid, the toxic substances benzoic acid, diisobutyl phthalate, diisobutyl succinate and 2,2- (4 - hydroxyphenyl) propane degradation solution on the growth of ginseng seeds Poisoning effect was significantly alleviated, no significant difference with the control. Except for benzoic acid, the activities of SOD, POD and CAT of ginseng after degraded by other autotoxic substances were significantly lower than those before degradation. The results show that microbial degradation is an effective way to alleviate the inhibition of self-toxic substances on germination of ginseng seeds, and this finding will help to solve the problem of continuous cropping obstacles.