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目的建立常见致病真菌的通用引物PCR检测技术,为研究致病真菌的基因芯片检测技术打下基础。方法以真菌的5.8SrRAN基因和28SrRAN基因为靶基因,利用其保守序列设计用于常见致病真菌检测的PCR通用引物,并观察检测方法的特异性及敏感性。结果建立的常见致病真菌通用引物PCR检测方法可有效检测白色念珠菌、新生隐球菌、烟曲霉、黄曲霉、黑曲霉、构巢曲霉等20个种属的致病真菌,敏感性为15pg/mlDNA,实际应用效果与标准菌株检测结果一致。结论本研究建立的通用引物PCR可以用于常见致病真菌的检测。
Objective To establish a universal primer PCR detection technology for common pathogenic fungi and lay the foundation for the study of gene chip detection technology of pathogenic fungi. Methods The 5.8SrRAN gene and 28SrRAN gene of fungi were used as target genes. Based on their conserved sequences, PCR primers were designed and used to detect common pathogenic fungi. The specificity and sensitivity of the method were observed. Results The common PCR primers for common pathogenic fungi were established to detect pathogenic fungi such as Candida albicans, Cryptococcus neoformans, Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger and Aspergillus nidulans with a sensitivity of 15 pg / ml DNA, the practical application of the results of the same strain of the test results. Conclusion The universal primer PCR established in this study can be used for the detection of common pathogenic fungi.