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为了研究同时测定饮料中八种添加剂 (柠檬黄、苋菜红、糖精钠、胭脂红、日落黄、苯甲酸、山梨酸、亮兰 )的高效液相色谱方法 (HPLC) ,应用高效液相色谱法 ,在ODS -C1 8柱上 ,对八种添加剂进行梯度洗脱分析。结果表明最佳色谱条件为 :检测波长 2 40nm ,柱温为 2 5°C ,流动相为甲醇———乙酸胺溶液 ( 0 .0 2mol/L ,pH =5 .0 ) ,甲醇洗脱梯度为 15 %— 2 5 % ,3 .3 3min ;2 5 %— 3 5 % ,5min ;3 5 %— 70 % ,7min ;70 %— 80 % ,5min ,流速 1ml/min。在此条件下 ,八种添加剂在 11分钟在内全部分离。标准曲线相关系数为R =0 .9998_0 .9999,检出限为 0 .15ug/ml— 2 .0ug/ml,精密度 (RSD)为 0 .3 8%— 1.2 9% ,试样加标回收率为 96.3 %— 10 5 .7% ,试样测定值本法与国标法无显著性差异。因此本法可同时准确地测定饮料中八种添加剂 ,适合基层卫生单位快速检测。
In order to study the HPLC method for the simultaneous determination of eight additives (lemon yellow, amaranth, sodium saccharin, carmine, sunset yellow, benzoic acid, sorbic acid, Brilliant Blue) in beverages, high performance liquid chromatography Eight eluents were run on a ODS-C8 column for gradient elution analysis. The results showed that the optimum chromatographic conditions were as follows: the detection wavelength was 2 40nm, the column temperature was 25 ℃, the mobile phase consisted of methanol-acetic acid amine solution (0.02 mol / L, pH = 5.0) 15% -25%, 3.3.3 min; 25% -35%, 5 min; 35% -70%, 7 min; 70% -80%, 5 min at a flow rate of 1 ml / min. Under these conditions, all eight additives were completely separated within 11 minutes. The correlation coefficient of standard curve was R = 0.9998_0.9999, the detection limit was 0.15ug / ml-2. 0ug / ml, the precision (RSD) was 0.38% -1.29% The rate of 96.3% - 105.7%, the measured value of the sample and the national standard method was no significant difference. Therefore, this method can simultaneously determine the eight kinds of additives in beverages, suitable for rapid detection of primary health units.