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[目的]探讨微波预先辐照对γ射线致骨髓基质细胞凋亡、线粒体膜电位、胞内游离Ca2+浓度和Ca2+-Mg2+-ATP酶活性改变的影响。[方法]将原代培养的骨髓基质细胞随机分为正常对照组、单纯微波组、单纯γ射线组和联合组。12μW/cm2微波对单纯微波组和联合组连续辐照7d,每天1h,第8天对单纯γ射线组和联合组进行5Gy的γ射线照射。用钙离子依赖性磷脂结合蛋白-异硫氰酸荧光素及碘化丙啶(AnnexinV-FITC及PI)双标记法检测细胞凋亡,流式细胞仪检测线粒体膜电位和胞内游离Ca2+浓度,试剂盒检测Ca2+-Mg2+-ATP酶活性。[结果]与正常对照组相比,各处理组细胞的凋亡率和线粒体膜电位未见明显改变。γ射线照射后24h,单纯微波组、联合组和单纯γ射线组细胞内游离Ca2+浓度明显升高(P<0.05),Ca2+-Mg2+-ATP酶活性明显降低(P<0.05)。与单纯γ射线组相比,联合组细胞内游离Ca2+浓度明显降低(P<0.05),Ca2+-Mg2+-ATP酶活性明显升高(P<0.05)。[结论]本实验条件下,900MHz,12μW/cm2的微波辐射和5Gyγ射线均不能引起细胞凋亡率、线粒体膜电位的明显变化,但能导致胞内游离Ca2+浓度明显上升和Ca2+-Mg2+-ATP酶活性明显降低。预先微波辐照能够明显减弱γ射线引起的胞内游离Ca2+浓度和Ca2+-Mg2+-ATP酶活性改变。
[Objective] To investigate the effect of microwave pre-irradiation on the apoptosis of bone marrow stromal cells, mitochondrial membrane potential, intracellular free Ca2 + concentration and Ca2 + -Mg2 + -ATPase activity induced by γ-rays. [Method] Primary cultured bone marrow stromal cells were randomly divided into normal control group, simple microwave group, simple γ-ray group and combined group. The microwaves of 12μW / cm2 were irradiated with 5Gy gamma rays for 7 days in the microwave group and the combination group for 1 day and 8 days in the pure γ-ray group and the combined group respectively. Cell apoptosis was detected by calcium-dependent phospholipid-binding protein-fluorescein isothiocyanate and propidium iodide (PI) double labeling method. Mitochondrial membrane potential and intracellular free Ca2 + concentration were detected by flow cytometry. Kit detects Ca2 + -Mg2 + -ATPase activity. [Result] Compared with the normal control group, the apoptosis rate and the mitochondrial membrane potential of all treatment groups showed no significant change. The concentration of intracellular free Ca2 + was significantly increased (P <0.05) and the activity of Ca2 + -Mg2 + -ATPase was decreased significantly (P <0.05) 24 h after γ ray irradiation in the microwave group, the combination group and the pure γ-ray group. Compared with the pure γ-ray group, the intracellular free Ca2 + concentration in the combined group was significantly decreased (P <0.05) and Ca2 + -Mg2 + -ATPase activity was significantly increased (P <0.05). [Conclusion] Under the experimental conditions, the radiation and 5Gy-γ radiation at 900MHz, 12μW / cm2 could not induce apoptosis and mitochondrial membrane potential changes, but could significantly increase intracellular free Ca2 + concentration and Ca2 + -Mg2 + -ATP Enzyme activity was significantly reduced. Pre-microwave irradiation can significantly reduce γ-ray-induced intracellular free Ca2 + concentration and Ca2 + -Mg2 + -ATPase activity changes.