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目的:观察牛磺酸对四氯化碳(CCl_4)诱导大鼠肝纤维化的保护及其机制.方法:采用CCl_4诱导的大鼠肝纤维化模型,研究牛磺酸(400 and 800 mg/kg per day for 12 weeks)对血清丙氨酸氨基转移酶(ALT)、血浆蛋白、透明质酸(HA)、Ⅲ型前胶原(PC Ⅲ)及肝组织羟脯氨酸(HYP)、肝药酶和抗转化生长因子β_1(TGF-β_1)的影响,并观察肝组织病理学变化.结果:牛磺酸能明显减轻纤维化程度,降低ALT(2 601±300→1367 ± 317 nkat/L,P<0.01),HA(324 ± 95→219 ± 79 μg/L,P<0.01),PC Ⅲ(33 ± 14→19± 8μg/L,P<0.01)和HYP(1 282 ± 523→381±147μg/L,P<0.01)水平,提高肝细胞色素P450(Cyt.P450)(211 ± 27→811±136 nmol/g,P<0.01),细胞色素b_5(Cyt.b_5)(256 ±69→761±131 nmol/g,P <0.1)和血清白蛋白含量(23±3→31 ±4 g/L,P<0.05),并明显减少TGF-β_1的表达(33±13→20 ± 6,P<0.01).结论:牛磺酸具有显著的抗CCl_4诱导的大鼠肝纤维化作用,其作用机制可能与减轻肝细胞损伤和抑制TGF-β_1的表达有关.
Objective: To observe the protective effect of taurine on hepatic fibrosis induced by carbon tetrachloride (CCl_4) and its mechanism.Methods: The model of hepatic fibrosis induced by CCl_4 was used to study the effects of taurine (400 and 800 mg / kg) per day for 12 weeks) on serum alanine aminotransferase (ALT), plasma protein, hyaluronic acid (HA), type Ⅲ procollagen (PC Ⅲ) and liver hydroxyproline (HYP) And TGF-β 1 (TGF-β 1) in the liver of the rats and observe the histopathological changes of the liver.Results: Taurine can obviously reduce the degree of fibrosis and reduce the level of ALT (2 601 ± 300 → 1367 ± 317 nkat / L, P (33 ± 14 → 19 ± 8 μg / L, P <0.01), HY (324 ± 95 → 219 ± 79 μg / L, P <0.01) (P <0.01). The levels of cytochrome b_5 (Cyt.b_5) (256 ± 69 → 761 ± 131 nmol / g, P <0.1) and serum albumin content (23 ± 3 → 31 ± 4 g / L, P <0.05) and significantly decreased the expression of TGF-β 1 (33 ± 13 → 20 ± 6, P <0.01) .Conclusion: Taurine has significant anti-CCl 4 -induced hepatic fibrosis in rats, which may be related to the reduction of hepatocellular injury And inhibit the expression of TGF-β_1.