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开发了一种多孔分子印迹膜修饰的表面等离子体共振(SPR)传感器,用于快速检测水中微囊藻毒素LR。研究了利用该传感器检测微囊藻毒素LR的方法。首先,通过原位聚合法在SPR传感芯片的裸金表面合成了微囊藻毒素LR的多孔分子印迹膜,制备出可以特异性捕获微囊藻毒素LR的SPR传感芯片。然后,利用Kretschmann棱镜耦合结构,构建了基于Kretschmann结构的波长调制型表面等离子体共振传感器。最后,通过检测不同浓度的微囊藻毒素LR溶液以及干扰物质微囊藻毒素RR溶液,研究了该传感器的测量范围、特异性等参数。结果表明,该传感器对于微囊藻毒素LR的检测灵敏度很高,可实现微囊藻毒素LR的定量检测,动态测量达2.1×10-9~1×10-6 mol/L。另外,传感器对于干扰物质微囊藻毒素RR无明显信号响应,表明传感器对于微囊藻毒素LR具有很好的特异性检测能力。
A porous molecularly imprinted membrane modified surface plasmon resonance (SPR) sensor was developed for the rapid detection of microcystin LR in water. The method for detecting microcystins LR using the sensor was studied. Firstly, the microtocytic toxin LR porous molecularly imprinted membrane was synthesized on the bare gold surface of the SPR sensor chip by in-situ polymerization to prepare an SPR sensor chip that can specifically capture microcystin LR. Then, based on the Kretschmann prism coupling structure, a wavelength modulated surface plasmon resonance sensor based on the Kretschmann structure is constructed. Finally, the detection range, specificity and other parameters of the sensor were studied by detecting different concentrations of microcystin LR solution and interfering substance microcystin RR solution. The results showed that the sensitivity of this sensor to microcystin LR was high, and the microcystin LR could be detected quantitatively with a dynamic measurement of 2.1 × 10-9 ~ 1 × 10-6 mol / L. In addition, the sensor has no obvious signal response to microcystin RR, which is the interfering substance, indicating that the sensor has good specific detection ability for microcystin LR.