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现在单克隆抗体已经广泛地被用来检测组织切片的细胞类型,通过免疫过氧化物酶技术可同时获得特异性染色及天然组织的形态学资料,并易于保存与拍照.但用辣根过氧化物酶(HRP)标记的兔抗小鼠血清对冰冻切片染色时,由于组织存在内源性过氧化物酶(EPX)活性,如果切片不经过冷冻干燥等处理,往往因特异性着色而影响本底清晰度.作者用腹部病人肠组织作冰冻切片,该组织EPX活性较高,通过筛选不同的EPX抑制剂,最后不进行冷冻干燥处理切片,同样消除染色的非特异
Now that monoclonal antibodies have been widely used to detect cell types in tissue sections, immunohistochemical staining for both histologically-specific and native tissue morphology can be easily performed and photographed, but the use of horseradish peroxidase When frozen sections are stained with HRP-labeled rabbit anti-mouse serum, endogenous peroxidase (EPX) activity is present in the tissue. If the sections are not subjected to freeze-drying and other treatments, the specific color often affects the Bottom sharpness The authors used frozen sections of intestinal tissue from abdomen patients, which showed high EPX activity, were screened for different EPX inhibitors, and were finally sectioned without freeze-drying to eliminate stained non-specific