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目的:探讨抗柯萨奇B病毒性心肌炎胶囊复方新制剂(K-CoxB-JN)对病毒性心肌炎小鼠血清肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)的影响及其机制。方法:用CVB3感染Balb/c小鼠造成病毒性心肌炎模型,随机分为5组:K-CoxBJN复方新制剂低、中、高剂量组(0.2,0.4,0.8 g·kg-1),利巴韦林组(0.075 g·kg-1),模型组,并设正常组,鼠腹腔接种病毒2 h后开始灌胃,连续灌胃14 d。观察小鼠一般状态、记录死亡数,计算小鼠生存率;取血测定血清TNF-α,IL-6水平;取心脏,计算心脏指数,HE染色,计算病理积分。结果:与正常组比较,模型组小鼠血清TNF-α,IL-6水平、心脏指数、心脏病理积分均升高(P<0.05),生存率降低(P<0.05);与模型组比较,K-CoxB-JN各组小鼠心脏指数,TNF-α,IL-6水平、心脏病理积分均降低(P<0.05),生存率升高(P<0.05)。结论:调节TNF-α,IL-6水平可能是K-CoxB-JN治疗病毒性心肌炎作用机制之一。
Objective: To investigate the effect of K-CoxB-JN on the levels of serum tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in mice with viral myocarditis And its mechanism. Methods: The models of viral myocarditis induced by CVB3 in Balb / c mice were randomly divided into 5 groups: low, medium and high doses of K-CoxBJN (0.2, 0.4 and 0.8 g · kg-1) Weilein group (0.075 g · kg-1), model group, and normal group. The mice were inoculated intraperitoneally with virus for 2 h and then intragastrically for 14 days. Observe the general state of mice, record the number of deaths, calculate the survival rate of mice; take blood to measure the level of serum TNF-α and IL-6; take the heart, calculate the cardiac index, HE staining, calculate the pathological score. Results: Compared with the normal group, the serum TNF-α and IL-6 levels, cardiac index and heart pathological scores of the model group were significantly increased (P <0.05) and the survival rate of the model group was lower than that of the normal group Cardiac index, TNF-α and IL-6 levels and heart pathological scores in K-CoxB-JN groups were decreased (P <0.05) and survival rate was increased (P <0.05). Conclusion: Regulating TNF-α and IL-6 levels may be one of the mechanisms of K-CoxB-JN treatment of viral myocarditis.