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目的:动脉粥样硬化是慢性炎症疾病,免疫细胞及炎症因子在其发病过程中起重要调节作用。本研究在于探讨IL-27对血管内皮细胞的直接作用。方法:采用ELISA方法,研究IL-27和TNF-α对人冠状动脉内皮细胞产生炎症相关细胞因子和趋化因子的作用。结果:IL-27显著增强TNF-α介导上调的炎症相关细胞因子IL-6和趋化因子CCL5的表达。IL-6和CCL5的表达受特异性信号分子抑制剂显著抑制。结论:人冠状动脉内皮细胞诱导释放IL-6和CCL5可能受JNK,p38MAPK和NF-κB通路差异调控。这些结果为阐明IL-27与TNF-α在动脉粥样硬化血管炎症发生中的作用提供重要生物化学基础。
AIM: Atherosclerosis is a chronic inflammatory disease. Immune cells and inflammatory cytokines play an important role in the pathogenesis. This study is to investigate the direct effect of IL-27 on vascular endothelial cells. Methods: The effects of IL-27 and TNF-α on the production of inflammatory cytokines and chemokines by human coronary artery endothelial cells were studied by ELISA. Results: IL-27 significantly enhanced the TNF-α-induced upregulation of inflammatory cytokines IL-6 and chemokine CCL5 expression. The expression of IL-6 and CCL5 was significantly inhibited by specific signaling molecule inhibitors. Conclusion: IL-6 and CCL5 induced by human coronary artery endothelial cells may be differentially regulated by JNK, p38MAPK and NF-κB pathway. These results provide an important biochemical basis for elucidating the role of IL-27 and TNF-a in the development of atherosclerotic vascular inflammation.