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本文研究了双氢青蒿素的β异构体与α异构体转化平衡条件；并采用反相高效液相色谱法对其进行定量测定。色谱条件为：ＹＷＧ－Ｃ１８色谱柱（２００ｍｍ×５ｍｍ，１０μｍ），乙腈－０．０２ｍｏ１／Ｌ硫酸铵溶液－１２％三乙胺溶液（６０：４０：０．２，ｐＨ５．０）为流动相，以萘作内标，检测波长为２１０ｎｍ，本法准确、简便、快速。
In this paper, the equilibrium conditions of β-isomer and α-isomer of dihydroartemisinin were studied, and the quantitative determination was carried out by reversed-phase high performance liquid chromatography. The chromatographic conditions were as follows: YWG-C18 column (200mm × 5mm, 10μm), acetonitrile-0.02mo1 / L ammonium sulfate solution -12% triethylamine solution (60:40: 0.2, pH5.0) , With naphthalene as internal standard, detection wavelength of 210nm, accurate, simple, fast.