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目的动态研究高碘与高酪氨酸对小鼠甲状腺形态结构变化的影响。方法将150只昆明种小鼠随机分为对照组(NI)、高碘组(HI)、高酪氨酸组(Htyr)、高碘高酪氨酸组(HI+Htyr)和高碘模型组(HIM)5组。在实验10周、20周和30周时,测定小鼠甲状腺绝对质量与相对质量,观察小鼠甲状腺组织形态变化。结果 20周时,HI组小鼠甲状腺相对质量显著高于NI组;30周时,HI+Htyr组小鼠甲状腺相对质量显著高于NI组。光镜结果显示,HI组呈现典型的胶质性甲状腺肿;同时HI+Htyr组也呈现出胶质性甲状腺肿的改变,但滤泡腔胶质减少。结论补充适量的酪氨酸可以减轻高碘对甲状腺组织的损伤作用,延缓昆明种小鼠甲肿的发生,但不能阻止高碘甲状腺肿的形成,说明在水碘为300μg/L的情况下,碘对甲状腺肿的作用是主要的,酪氨酸的作用是次要的。
Objective To investigate the effects of high iodine and high tyrosine on the changes of thyroid morphology and structure in mice. Methods 150 Kunming mice were randomly divided into control group (NI), high iodine group (HI), high tyrosine group (Htyr), high iodine tyrosine group (HI + Htyr) and high iodine model group (HIM) 5 groups. At 10, 20 and 30 weeks of experiment, the absolute mass and the relative quality of thyroid in mice were determined, and the morphological changes of thyroid in mice were observed. Results At 20 weeks, the relative quality of thyroid in HI group was significantly higher than that in NI group. At 30 weeks, the relative quality of thyroid in HI + Htyr group was significantly higher than that in NI group. Light microscopy results showed that HI group showed a typical glial goiter; also HI + Htyr group also showed a change of gliacyte goiter, but the follicular cavity glial decreased. Conclusion Supplementation of appropriate amount of tyrosine can reduce the damage of thyroid tissue induced by high iodine, delay the occurrence of thyrotrophosis in Kunming mice, but can not prevent the formation of high iodine goiter, indicating that in the case of water iodine 300μg / L, The effect of iodine on goiter is major, the role of tyrosine is secondary.