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Hydrolysis of DNA is an important enzymatic reaction , but it is exceedingly difficult to mimic in the laboratory because of the stability of hydrolysis of DNA. In this paper, the cleavage activity of complexes formed between Cu(Ⅱ) and four different amino acid or amino acid methyl ester on DNA is studied by gel elec-trophoresis. It is found that DNA could be cleaved by Cu(Ⅱ)-L-His and Cu(Ⅱ)-L-His methyl ester complexes and the efficiency of cleavage is largely dependent on the metal ion-to-ligand ratio. Further experiments show that the cleavage of DNA mediated by Cu(Ⅱ)-L-His complexes occurs via a hydrolytic mechanism and the active chemical species that affects DNA cleavage is proposed to be MI2H+ and ML2H22+.
Hydrolysis of DNA is an important enzymatic reaction, but it is exceedingly difficult to mimic in the laboratory because of stability of hydrolysis of DNA. In this paper, the cleavage activity of complexes forming between Cu (II) and four different amino acid or amino It is found that DNA could be cleaved by Cu (II) -L-His and Cu (II) -L-His methyl ester complexes and the efficiency of cleavage is largely dependent Further experiments show that the cleavage of DNA mediated by Cu (II) -L-His complexes occurs via a hydrolytic mechanism and the active chemical species that affects DNA cleavage is proposed to MI2H + and ML2H22 + .