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以MS为基本培养基,分别添加IAA0.05—1.0mg/l、BA0.2—8.0mg/l、KT0.2—2.0mg/l和PAA0.1—10.0mg/l的激素,对枸杞无菌苗子叶和下胚轴进行离体培养,所产生的愈伤组织可在原培养基上直接分化出芽,其中子叶愈伤组织的平均诱导频率、分化频率和每块愈伤组织直接产生的芽数均高于下胚轴。
To MS as the basic medium were added IAA0.05-1.0mg / l, BA0.2-8.0mg / l, KT0.2-2.0mg / l and PAA0.1-10.0mg / l of the hormone, wolfberry without The cotyledons and hypocotyls of the seedlings were cultured in vitro. The callus generated could differentiate directly on the original culture medium. The average induction frequency, the frequency of differentiation and the number of shoots per callus Higher than hypocotyls.