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为了观察李氏5号方对半乳糖老化小鼠海马神经元褪黑素受体(MTR)和Nogo受体(NogoR)表达的影响。我们将昆明小鼠随机分为五组:正常对照组(C组)、D半乳糖模型组(D组)、李氏5号方大剂量药物治疗组(L组)、中剂量药物治疗组(M组)和小剂量药物治疗组(S组);D、L、M、S四组每日皮下注射半乳糖65mg/kg体重,持续三个月。应用免疫组织化学染色方法检测小鼠海马神经元MTR和NogoR的表达水平。NogoR免疫组化染色结果:D组海马阳性反应细胞着色浅,少有突起,细胞数目少,仅为正常小鼠的20%,三个剂量李氏5号方治疗组染色结果与C组相似。结果提示:半乳糖老化小鼠海马神经元MTR免疫反应阳性神经元数的上调可能是老化小鼠的一种代偿性反应;NogoR表达下调提示老化小鼠可能存在脑白质的损害,出于机体自我保护的需要,NogoR表达下调以避免神经元修复机制对大脑的进一步损害;不同剂量的李氏5号方水提取液对两种受体的调节作用极为明显,说明这两种受体的表达可受到外源性药物的干预。
To observe the effect of Lishi 5 on the expression of melatonin receptor (MTR) and Nogo receptor (NogoR) in hippocampal neurons of galactose-induced aging mice. We randomly divided Kunming mice into five groups: normal control group (C group), D galactose model group (D group), Lee’s No.5 high-dose drug treatment group (L group), and medium-dose drug treatment group ( M group) and low-dose drug treatment group (S group); D, L, M, S four groups of daily subcutaneous injection of galactose 65mg/kg body weight for three months. Immunohistochemical staining was used to detect the expression of MTR and NogoR in mouse hippocampal neurons. NogoR immunohistochemical staining results: D group hippocampus positive reaction cells were lightly colored, there are few protrusions, the number of cells is only 20% of normal mice, the staining results of the three doses of the Lee’s No. 5 prescription treatment group are similar to the C group. The results suggest that the upregulation of MTR immunoreactive neurons in hippocampal neurons of galactose-aged mice may be a compensatory response in aging mice; down-regulation of NogoR suggests that aging mice may have white matter damage due to the body The need for self-protection, NogoR expression downregulated to avoid further damage to the brain by neuronal repair mechanisms; different doses of Lee’s No. 5 water extract on the regulation of the two receptors is extremely obvious, indicating the expression of these two receptors Can be interfered with by exogenous drugs.