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目的 探索瑞香狼素 (SC)抗肿瘤机制。方法 以 HL- 6 0和 SGC- 790 1为靶细胞 ,用 MTT比色法测定细胞增殖抑制 ,荧光显微镜观察凋亡细胞的形态学改变 ,DNA电泳和流式细胞仪检测DNA断裂 ,免疫组化检测 bcl- 2蛋白表达。结果 含 SC药物血清处理细胞 48h后 ,HL- 6 0细胞增殖呈剂量依赖性抑制 ,并表现出典型的凋亡细胞形态学改变及 DNA断裂 :即染色体聚集、核固缩、断裂及阶梯状 DNA电泳条带 ,G1 期前细胞从 11.7%增至 5 7.4% ;而 SGC- 790 1细胞 bcl- 2蛋白表达率从 78.3%下降到 32 .9%。结论 SC可诱导肿瘤细胞凋亡 ,降低 bcl- 2蛋白表达。
Objective To explore the anti-tumor mechanism of Seikalin (SC). Methods HL-60 and SGC-790 1 were used as target cells. MTT colorimetric assay was used to determine the inhibition of cell proliferation. Morphological changes of apoptotic cells were observed by fluorescence microscope. DNA fragmentation and DNA fragmentation were detected by flow cytometry and immunohistochemistry. Detection of bcl-2 protein expression. Results The proliferation of HL-60 cells was inhibited in a dose-dependent manner after treatment with SC drug serum for 48 h, and typical morphological changes of apoptotic cells and DNA fragmentation were observed: chromosomal aggregation, nuclear condensation, breakage, and ladder-like DNA The electrophoretic bands increased from 11.7% to 5 7.4% before G1 phase, while the expression of bcl-2 protein decreased from 78.3% to 32.9% in SGC-7901 cells. Conclusion SC can induce apoptosis and reduce the expression of bcl-2 protein.