Objective: The aim of this study was to analyze the expression of CC chemokine receptor 7 (CCR7) in pulmo-nary tumor tissue and metastasized lymph nodes of non-small cell lung cancer (NSCLC), explore the relationship between the expressions of CCR7 in pulmonary tumor tissue and metastasized lymph nodes, and discuss the significance. Methods: SABC immunohistochemical staining was used to investigate the expression of CCR7 by rabbit anti-human CCR7 mono-clonal antibody, and the specimens were 17 cases of adenocarcinoma, 17 cases of squamous cell carcinoma, 12 cases of adenosquamous carcinoma, 4 cases of large cell carcinoma and 28 cases of metastasized lymph nodes of lung cancer. Negative control sections used 5 cases of inflammatory pseudotumor and 20 cases of normal lung tissue. Two independent pathologists observed all the specimens in the high power field (x 400) of microscope by double blind method. Results: (1) The expression of CCR7 in pulmonary tumor tissue was stronger than normal lung tissue (P < 0.005); (2) The expressions of CCR7 in pulmonary tumor tissues and metastasized lymph nodes had no significant differences (P=0.177); (3) The expres-sion of CCR7 had correlation with lymph nodes metastasis, and the expression in lymph nodes metastasis group was more than that of no lymph nodes metastasis group (P=0.016); (4) Along with the increment of clinical stage, the CCR7 expression had a tendency to increase (P=0.003). Conclusion: CCR7 has rich expression in carcinoma cell nests and lymph node metastasis, it demonstrates that CCR7 may be related to the development of lymph node metastasis in NSCLC.