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目的:观察补中益气汤水提物及其乙酸乙酯萃取部分对大鼠小肠平滑肌动力的影响。方法:采用大鼠离体小肠平滑肌的幅值、频率和基线变化为观察指标,比较补中益气汤水提物及其乙酸乙酯萃取物对离体肠肌自主收缩运动的影响。结果:补中益气汤水提物能明显抑制大鼠离体小肠平滑肌收缩的幅值和基线,明显增加收缩的频率,均有统计学意义(P<0.05);补中益气汤水提物的乙酸乙酯萃取物也具有相似的效果。另外,补中益气汤水提物及其乙酸乙酯萃取物均可缓解乙酰胆碱引起的肠肌痉挛。结论:补中益气汤水提物的乙酸乙酯萃取物对离体小肠平滑肌的作用类似于原复方水提物,该部分是补中益气汤复方的主要有效部位。
Objective: To observe the effects of Buzhong Yiqi decoction and its ethyl acetate extract on the dynamic of small intestine smooth muscle in rats. Methods: The amplitude, frequency and baseline changes of isolated rat intestinal smooth muscle were used as observation indexes to compare the effects of Buzhong Yiqi decoction extract and ethyl acetate extract on contractile contraction of isolated intestinal muscle. Results: The water extract from Buzhong Yiqi decoction could significantly inhibit the contractile amplitude and baseline of isolated rat intestinal smooth muscle and significantly increase the frequency of contractions (P <0.05) Ethyl acetate extracts also have similar effects. In addition, Buzhong Yiqi soup extract and ethyl acetate extract can relieve acetylcholine-induced intestinal muscle spasm. Conclusion: The ethyl acetate extracts of Buzhong Yiqi Decoction extract have the same effect on the isolated small intestine smooth muscle as the original compound aqueous extract, which is the main effective part of Buzhong Yiqi Decoction.