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检测骨髓增生异常综合征(MDS)和再生障碍性贫血(AA)患者骨髓细胞免疫表型特征,探讨其对二者发病机制、分型诊断及鉴别诊断的临床意义。选用多种单克隆抗体,应用直接免疫荧光法采用流式细胞术CD45/SSC设门,对23例MDS、14例AA、9例正常对照的骨髓细胞各免疫标志的表达率进行检测。结果:MDS组与正常对照组相比其造血干/祖细胞抗原CD34、HLA-DR、髓系早期抗原CD13、CD33、单核系抗原CD14、T淋巴细胞抗原CD7的表达率增高,髓系成熟抗原CD15、B淋巴系抗原CD19、CD20的表达率降低,DI值增高;由RA进展至RAEB,CD34、HLA-DR、CD13、CD33、CD14、CD7的表达率逐渐增高,CD15、CD3、CD19、CD20的表达率逐渐减低,DI值逐渐增高。AA组与正常对照组相比其CD34、CD13、CD33、CD15的表达率降低,CD3、CD7、CD25、CD22的表达率增高。MDS组与AA组相比其CD34、HLA-DR、CD13、CD33、CD14的表达率增高,CD3、CD5、CD7、CD15、CD19、CD20、CD22、CD25的表达率显著降低。MDS和AA患者骨髓细胞免疫表型分析,有助于揭示二者的发病机制,为临床提供新的诊断、分型及鉴别诊断方法。
To detect the immunophenotypic characteristics of bone marrow cells in patients with myelodysplastic syndrome (MDS) and aplastic anemia (AA), and to explore its clinical significance in the pathogenesis, classification and differential diagnosis of both. A variety of monoclonal antibodies were used to detect the expression of various immune markers in 23 cases of MDS, 14 cases of AA, 9 cases of normal control bone marrow cells by flow cytometry CD45 / SSC using direct immunofluorescence. Results: Compared with the normal control group, the expression of CD34, HLA-DR, myeloid early antigen CD13, CD33, monocyte-derived antigen CD14 and T lymphocyte antigen CD7 in MDS group was higher than that in normal control group The expressions of CD15, CD3, CD19, CD19, CD19, CD19, CD19, CD19, The expression of CD20 gradually decreased and DI value gradually increased. Compared with the normal control group, the expression of CD34, CD13, CD33 and CD15 in AA group decreased and the expression rates of CD3, CD7, CD25 and CD22 increased. The expression of CD34, HLA-DR, CD13, CD33 and CD14 in MDS group was significantly higher than that in AA group. The expression rates of CD3, CD5, CD7, CD15, CD19, CD20, CD22 and CD25 in MDS group were significantly decreased. Immunophenotypic analysis of bone marrow cells in patients with MDS and AA will help reveal the pathogenesis of both and provide new diagnostic, classification and differential diagnosis methods for clinical diagnosis.