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目的研究中国流行的轮状病毒(Rotavirus,RV)G9型毒株的分子流行病学特征。方法在中国9个地区收集5岁以下腹泻患儿粪便标本,应用酶联免疫吸附试验(ELISA)方法检测RV,对阳性标本用逆转录-聚合酶链反应(RT-PCR)分型,选择G9型毒株进行VP7基因全长克隆测序和分子流行病学分析。结果1998~2004年共检测出RV1 268份,其中45份为G9型(3.5%),昆明最多(34/45),其次是兰州(8/45)、长春(2/45)、卢龙(1/45),北京、郑州、杭州、福州、广州未检测到G9型毒株。对35份G9型标本进行P分型鉴定:15份为P[8]型,12份为P[6]型,5份为P[4]型,1份为P[8+4]混合型,2份未能分型。中国1998~2000年以P[8]G9毒株流行为主,而2001年后以P[6]G9毒株为主。22株G9型病毒VP7基因序列比对结果表明,中国流行的G9型毒株彼此同源性高,同属G9第3亚型。结论中国流行的RV G9型株与世界各地的流行株同源性较高,国内传播范围扩大的趋势值得关注。
Objective To study the molecular epidemiology of G9 strain of rotavirus (RV) in China. Methods Stool samples from children with diarrhea under 5 years of age were collected in 9 districts of China. RV was detected by enzyme-linked immunosorbent assay (ELISA), and RT-PCR was used to detect the positive samples. G9 Type strain was cloned and sequenced by molecular epidemiology. Results A total of 268 RV1 were detected in 1998-2004, of which 45 were G9 (3.5%), Kunming (34/45), followed by Lanzhou (8/45), Changchun 1/45). No G9 strain was detected in Beijing, Zhengzhou, Hangzhou, Fuzhou and Guangzhou. P type identification was performed on 35 G9 specimens: 15 were P [8], 12 were P [6], 5 were P [4] and 1 was P [8 + 4] , 2 failed to type. In China from 1998 to 2000, P [8] G9 strains were predominant, while P [6] G9 strains predominated after 2001. Sequence comparison of 22 strains of G9 virus VP7 showed that the G9 strains that are popular in China shared high homology with each other and belonged to G9 type 3 subtype. Conclusion The prevalence of RV G9 strain in China is high in homology with the epidemic strains in other parts of the world.