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目的 研究布比卡因对离体大鼠心脏功能和心肌能量代谢的影响。方法 Langendroff心脏灌注模型。2 4只SD大鼠随机分为对照组、6 μmol/L布比卡因组 (B1组 )和 12 μmol/L布比卡因组 (B2 组 ) ,每组 8只。B1、B2 组在平衡灌注 30min后 ,分别续灌含 6 μmol/L、12 μmol/L布比卡因的K -H液 ,记录上述各组在平衡末、用药 5min、10min、15min、2 5min时的HR、LVDP、DP、+dp/dt、-dp/dt,灌注 2 5min后立即取左室前壁心肌测定ATP含量。对照组平衡 30min后继续用K -H液灌注 2 5min ,测定相应时间点各项指标。结果 B1、B2 组用药后心率均较用药前明显降低 ,2组心率最大下降幅度分别为 2 4%和 5 1% (P <0 .0 1)。B1组用药后的LVDP、DP、+dp/dt、-dp/dt较用药前明显降低 ,B2 组用药后 10min、15min、2 5min时的LVDP较用药前明显升高 ,但用药后DP、+dp/dt、-dp/dt较用药前明显降低。B2 组心肌ATP含量明显低于B1组和对照组 (P <0 .0 1) ,B1组和对照组之间无显著性差异。结论 布比卡因对心率和心肌收缩功能均有明显的抑制作用 ,其抑制心肌收缩力的作用可能与线粒体能量代谢障碍有关
Objective To study the effects of bupivacaine on cardiac function and myocardial energy metabolism in isolated rat hearts. Methods Langendroff cardiac perfusion model. 24 SD rats were randomly divided into control group, 6 μmol / L bupivacaine group (B1 group) and 12 μmol / L bupivacaine group (B2 group), with 8 rats in each group. B1 and B2 groups were perfused with K-H solution containing 6 μmol / L and 12 μmol / L bupivacaine respectively after 30 min of equilibration. The above groups were treated with 5 min, 10 min, 15 min and 25 min The contents of ATP in the anterior wall of the left ventricle were determined immediately after HR, LVDP, DP, + dp / dt and -dp / dt, respectively. The control group continued to use K-H solution for 25 min after the equilibration for 30 min, and the indexes of the corresponding time points were determined. Results The heart rate of B1 and B2 groups were significantly lower than those before treatment, and the maximum decrease of heart rate was 24% and 51% respectively (P <0.01). The LVDP, DP, dp / dt and -dp / dt in group B1 were significantly lower than those before treatment. The LVDP in group B2 was significantly higher than that before treatment at 10min, 15min and 25min, dp / dt, -dp / dt was significantly lower than before treatment. The content of ATP in myocardium of B2 group was significantly lower than that of B1 group and control group (P <0.01). There was no significant difference between B1 group and control group. Conclusion Bupivacaine has significant inhibitory effect on heart rate and myocardial contractility, and its inhibitory effect on myocardial contractility may be related to the energy metabolism disorder of mitochondria